Descriptive study of perineuronal net in enteric nervous system of humans and mice.

Autor: da Silva MDV; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., Bacarin CC; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Machado CCA; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Franciosi A; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., Mendes JDL; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., da Silva Watanabe P; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Miqueloto CA; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Fattori V; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil.; Vascular Biology Program, Boston Children's Hospital, Department of Surgery, Harvard Medical School, Boston, Massachusetts, USA., Albarracin OYE; Production Engineering, Department of Engineering, Mackenzie Presbyterian University, São Paulo, São Paulo, Brazil., Verri WA Jr; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., Aktar R; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Peiris M; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Aziz Q; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Blackshaw LA; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., de Almeida Araújo EJ; Laboratory of Enteric Neuroscience, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Journal of neurochemistry [J Neurochem] 2024 Sep; Vol. 168 (9), pp. 1956-1972. Date of Electronic Publication: 2024 Jul 06.
DOI: 10.1111/jnc.16159
Abstrakt: Perineuronal nets (PNN) are highly specialized structures of the extracellular matrix around specific groups of neurons in the central nervous system (CNS). They play functions related to optimizing physiological processes and protection neurons against harmful stimuli. Traditionally, their existence was only described in the CNS. However, there was no description of the presence and composition of PNN in the enteric nervous system (ENS) until now. Thus, our aim was to demonstrate the presence and characterize the components of the PNN in the enteric nervous system. Samples of intestinal tissue from mice and humans were analyzed by RT-PCR and immunofluorescence assays. We used a marker (Wisteria floribunda agglutinin) considered as standard for detecting the presence of PNN in the CNS and antibodies for labeling members of the four main PNN-related protein families in the CNS. Our results demonstrated the presence of components of PNN in the ENS of both species; however its molecular composition is species-specific.
(© 2024 International Society for Neurochemistry.)
Databáze: MEDLINE
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