Diagnosis of pediatric central nervous system tumors using methylation profiling of cfDNA from cerebrospinal fluid.

Autor: Cornelli L; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Center for Medical Biotechnology, VIB-UGent, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium., Van Paemel R; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium.; Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, Ghent, Belgium., Ferro Dos Santos MR; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Center for Medical Biotechnology, VIB-UGent, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium., Roelandt S; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Center for Medical Biotechnology, VIB-UGent, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium., Willems L; Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Vandersteene J; Department of Neurosurgery, Ghent University Hospital, Ghent, Belgium., Baert E; Department of Neurosurgery, Ghent University Hospital, Ghent, Belgium., Mus LM; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium.; Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, Ghent, Belgium., Van Roy N; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium., De Wilde B; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium.; Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., De Preter K; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium. katleen.depreter@ugent.be.; Center for Medical Biotechnology, VIB-UGent, Ghent, Belgium. katleen.depreter@ugent.be.; Cancer Research Institute Ghent, Ghent, Belgium. katleen.depreter@ugent.be.
Jazyk: angličtina
Zdroj: Clinical epigenetics [Clin Epigenetics] 2024 Jul 05; Vol. 16 (1), pp. 87. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1186/s13148-024-01696-w
Abstrakt: Pediatric central nervous system tumors remain challenging to diagnose. Imaging approaches do not provide sufficient detail to discriminate between different tumor types, while the histopathological examination of tumor tissue shows high inter-observer variability. Recent studies have demonstrated the accurate classification of central nervous system tumors based on the DNA methylation profile of a tumor biopsy. However, a brain biopsy holds significant risk of bleeding and damaging the surrounding tissues. Liquid biopsy approaches analyzing circulating tumor DNA show high potential as an alternative and less invasive tool to study the DNA methylation pattern of tumors. Here, we explore the potential of classifying pediatric brain tumors based on methylation profiling of the circulating cell-free DNA (cfDNA) in cerebrospinal fluid (CSF). For this proof-of-concept study, we collected cerebrospinal fluid samples from 19 pediatric brain cancer patients via a ventricular drain placed for reasons of increased intracranial pressure. Analyses on the cfDNA showed high variability of cfDNA quantities across patients ranging from levels below the limit of quantification to 40 ng cfDNA per milliliter of CSF. Classification based on methylation profiling of cfDNA from CSF was correct for 7 out of 20 samples in our cohort. Accurate results were mostly observed in samples of high quality, more specifically those with limited high molecular weight DNA contamination. Interestingly, we show that centrifugation of the CSF prior to processing increases the fraction of fragmented cfDNA to high molecular weight DNA. In addition, classification was mostly correct for samples with high tumoral cfDNA fraction as estimated by computational deconvolution (> 40%). In summary, analysis of cfDNA in the CSF shows potential as a tool for diagnosing pediatric nervous system tumors especially in patients with high levels of tumoral cfDNA in the CSF. Further optimization of the collection procedure, experimental workflow and bioinformatic approach is required to also allow classification for patients with low tumoral fractions in the CSF.
(© 2024. The Author(s).)
Databáze: MEDLINE
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