Design of a novel multi-epitope vaccine against Marburg virus using immunoinformatics studies.

Autor: Al-Zayadi FQJ; Department of Biology, College of Education for Pure Sciences, Al-Muthanna University, Al-Muthanna, Iraq., Shakir AS; College of Dentistry, University of Al-Qadisiyah, Diwaniyah, Iraq., Kareem AS; Department of Medical Laboratories Techniques, Imam Ja'afar Al-Sadiq University, Al-Muthanna, Babylon, 66002, Iraq., Ghasemian A; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran. majidghasemian86@gmail.com., Behmard E; School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran. behmard62@gmail.com.
Jazyk: angličtina
Zdroj: BMC biotechnology [BMC Biotechnol] 2024 Jul 05; Vol. 24 (1), pp. 45. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1186/s12896-024-00873-2
Abstrakt: Marburg virus (MARV) is a highly contagious and virulent agent belonging to Filoviridae family. MARV causes severe hemorrhagic fever in humans and non-human primates. Owing to its highly virulent nature, preventive approaches are promising for its control. There is currently no approved drug or vaccine against MARV, and management mainly involves supportive care to treat symptoms and prevent complications. Our aim was to design a novel multi-epitope vaccine (MEV) against MARV using immunoinformatics studies. In this study, various proteins (VP35, VP40 and glycoprotein precursor) were used and potential epitopes were selected. CTL and HTL epitopes covered 79.44% and 70.55% of the global population, respectively. The designed MEV construct was stable and expressed in Escherichia coli (E. coli) host. The physicochemical properties were also acceptable. MARV MEV candidate could predict comprehensive immune responses such as those of humoral and cellular in silico. Additionally, efficient interaction to toll-like receptor 3 (TLR3) and its agonist (β-defensin) was predicted. There is a need for validation of these results using further in vitro and in vivo studies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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