Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.

Autor: Konold PE; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Monrroy L; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden., Bellisario A; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Filipe D; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Adams P; School of Science, STEM College, RMIT University, Melbourne, Victoria, Australia., Alvarez R; Department of Physics, Arizona State University, Tempe, AZ, USA., Bean R; European XFEL, Schenefeld, Germany., Bielecki J; European XFEL, Schenefeld, Germany., Bódizs S; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden., Ducrocq G; Department of Computer and Information Science (IDA), Linköping University, Linköping, Sweden.; The Division of Statistics and Machine Learning (STIMA), Linköping University, Linköping, Sweden., Grubmueller H; Department of Computer and Information Science (IDA), Linköping University, Linköping, Sweden., Kirian RA; European XFEL, Schenefeld, Germany., Kloos M; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden., Koliyadu JCP; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden., Koua FHM; European XFEL, Schenefeld, Germany., Larkiala T; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden., Letrun R; European XFEL, Schenefeld, Germany., Lindsten F; Department of Computer and Information Science (IDA), Linköping University, Linköping, Sweden.; The Division of Statistics and Machine Learning (STIMA), Linköping University, Linköping, Sweden., Maihöfer M; Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany., Martin AV; School of Science, STEM College, RMIT University, Melbourne, Victoria, Australia., Mészáros P; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden., Mutisya J; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden., Nimmrich A; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.; Department of Chemistry, University of Washington, Seattle, WA, USA., Okamoto K; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Round A; European XFEL, Schenefeld, Germany., Sato T; European XFEL, Schenefeld, Germany., Valerio J; European XFEL, Schenefeld, Germany., Westphal D; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Wollter A; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Yenupuri TV; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., You T; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Maia F; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden. filipe.maia@icm.uu.se., Westenhoff S; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden. sebastian.westenhoff@kemi.uu.se.; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. sebastian.westenhoff@kemi.uu.se.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2024 Sep; Vol. 21 (9), pp. 1608-1611. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1038/s41592-024-02344-0
Abstrakt: Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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