The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germination.

Autor:	Otani M; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.; Organization for the Strategic Coordination of Research and Intellectual Properties, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan., Tojo R; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan., Regnard S; Institute of Plant Sciences Paris Saclay, Paris-Saclay University, CNRS, National Research Institute for Agriculture, Food and the Environment (INRAE), Paris-Cité University, Evry Val d'Essonne University, Gif-sur-Yvette 91190, France., Zheng L; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.; Institute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei 230031, China.; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Anhui 230027, China., Hoshi T; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan., Ohmori S; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan., Tachibana N; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan., Sano T; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan., Koshimizu S; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.; Bioinformation and DDBJ Center, National Institute of Genetics, Mishima 411-8540, Japan., Ichimura K; Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan., Colcombet J; Institute of Plant Sciences Paris Saclay, Paris-Saclay University, CNRS, National Research Institute for Agriculture, Food and the Environment (INRAE), Paris-Cité University, Evry Val d'Essonne University, Gif-sur-Yvette 91190, France., Kawakami N; Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jul 09; Vol. 121 (28), pp. e2404887121. Date of Electronic Publication: 2024 Jul 05.
DOI:	10.1073/pnas.2404887121
Abstrakt:	The timing of seed germination is controlled by the combination of internal dormancy and external factors. Temperature is a major environmental factor for seed germination. The permissive temperature range for germination is narrow in dormant seeds and expands during after-ripening (AR) (dormancy release). Quantitative trait loci analyses of preharvest sprouting in cereals have revealed that MKK3, a mitogen-activated protein kinase (MAPK) cascade protein, is a negative regulator of grain dormancy. Here, we show that the MAPKKK19/20-MKK3-MPK1/2/7/14 cascade modulates the germination temperature range in Arabidopsis seeds by elevating the germinability of the seeds at sub- and supraoptimal temperatures. The expression of MAPKKK19 and MAPKKK20 is induced around optimal temperature for germination in after-ripened seeds but repressed in dormant seeds. MPK7 activation depends on the expression levels of MAPKKK19/20 , with expression occurring under conditions permissive for germination. Abscisic acid (ABA) and gibberellin (GA) are two major phytohormones which are involved in germination control. Activation of the MKK3 cascade represses ABA biosynthesis enzyme gene expression and induces expression of ABA catabolic enzyme and GA biosynthesis enzyme genes, resulting in expansion of the germinable temperature range. Our data demonstrate that the MKK3 cascade integrates temperature and AR signals to phytohormone metabolism and seed germination. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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