Hydroxyproline Concentration and Associated Factors of Preserved Small Incision Lenticule Extraction-Derived Corneal Stromal Lenticules.

Autor:	Han X; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China ., Li M; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China ., Zhao J; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China ., Sun B; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China ., Zhang X; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China ., Xu H; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China ., Zhou X; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China ; and.; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, China .
Jazyk:	angličtina
Zdroj:	Cornea [Cornea] 2024 Oct 01; Vol. 43 (10), pp. 1285-1290. Date of Electronic Publication: 2024 Jul 05.
DOI:	10.1097/ICO.0000000000003615
Abstrakt:	Purpose: To evaluate changes of hydroxyproline concentration and its influencing factors of small incision lenticule extraction (SMILE)-derived corneal stromal lenticules with different preservation methods. Methods: A total of 390 corneal stromal lenticules of 195 patients were derived from SMILE surgeries. Thirty of the lenticules were classified as the fresh (control) group, and the rest were randomly and evenly divided and stored in anhydrous glycerol, silicone oil, Optisol, and cryopreservation for 1 day, 1 week, or 1 month. A hydroxyproline assay kit (ab222941, Abcam) was used to measure the hydroxyproline concentration in each preservation method. Concentrations of MMP-2, TIMP-2, TNFα, TGFβ2, and reactive oxygen species were also evaluated. Results: In the anhydrous glycerol group, the concentration of hydroxyproline decreased within 1 week (fresh: 1 dΔ = 0.229, P < 0.001*; 1 d - 1 wΔ = 0.055, P < 0.001*) while that in the silicone oil group remained stable in 1 week (1 d - 1 wΔ = -0.005, P = 0.929) and decreased significantly in 1 m (1 m - 1 wΔ = -0.041, P = 0.003*). The sequence of hydroxyproline concentration in the Optisol group was 1 m > 1 day > 1 week. Hydroxyproline concentration in the cryopreservation group decreased within 1 m. Hydroxyproline concentration was highest in the Optisol group and lowest in the anhydrous glycerol group under the same preservation time. Hydroxyproline concentration was negatively correlated with MMP-2 (r = -0.16, P = 0.421) and TIMP-2 (r = -0.56, P = 0.002*) while MMP-2 and TNFα (r = 0.17, P = 0.242), TIMP-2 and TGFβ2 (r = 0.21, P = 0.207), and TNFα and reactive oxygen species (r = 0.52, P = 0.007*) were positively correlated. Conclusions: More collagen was retained in SMILE lenticules preserved in Optisol under the same preservation time. The mechanism of the changes of collagen in preserved SMILE-derived lenticules and oxidative stress requires additional investigation. Competing Interests: The authors have no conflicts of interest to disclose. (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu