Diversification of the Rho transcription termination factor in bacteria.

Autor:	Moreira SM; Department of Biochemistry, University of Otago, Dunedin, Otago 9054, New Zealand., Chyou TY; Department of Biochemistry, University of Otago, Dunedin, Otago 9054, New Zealand., Wade JT; Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA.; Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12222, USA., Brown CM; Department of Biochemistry, University of Otago, Dunedin, Otago 9054, New Zealand.; Genetics Otago, University of Otago, Dunedin, Otago 9054, New Zealand.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2024 Aug 27; Vol. 52 (15), pp. 8979-8997.
DOI:	10.1093/nar/gkae582
Abstrakt:	Correct termination of transcription is essential for gene expression. In bacteria, factor-dependent termination relies on the Rho factor, that classically has three conserved domains. Some bacteria also have a functional insertion region. However, the variation in Rho structure among bacteria has not been analyzed in detail. This study determines the distribution, sequence conservation, and predicted features of Rho factors with diverse domain architectures by analyzing 2730 bacterial genomes. About half (49.8%) of the species analyzed have the typical Escherichia coli like Rho while most of the other species (39.8%) have diverse, atypical forms of Rho. Besides conservation of the main domains, we describe a duplicated RNA-binding domain present in specific species and novel variations in the bicyclomycin binding pocket. The additional regions observed in Rho proteins exhibit remarkable diversity. Commonly, however, they have exceptional amino acid compositions and are predicted to be intrinsically disordered, to undergo phase separation, or have prion-like behavior. Phase separation has recently been shown to play roles in Rho function and bacterial fitness during harsh conditions in one species and this study suggests a more widespread role. In conclusion, diverse atypical Rho factors are broadly distributed among bacteria, suggesting additional cellular roles. (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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