Exploration of gut microbial diversity of pheasants through pyrosequencing of 16S rRNA gene.

Autor: Bukhari SM; Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan., Andleeb S; Department of Environmental Sciences, Faculty of Natural Sciences, GC Women University, Sialkot, Pakistan., Alghamdi HA; Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia., Rehman KU; Department of Environmental Sciences, Faculty of Natural Sciences, GC Women University, Sialkot, Pakistan., Javid A; Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan., Ali W; Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan., Abbas Y; Department of Animal Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan., Nokhaiz K; Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Jazyk: angličtina
Zdroj: Journal of experimental zoology. Part A, Ecological and integrative physiology [J Exp Zool A Ecol Integr Physiol] 2024 Oct; Vol. 341 (8), pp. 950-959. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1002/jez.2855
Abstrakt: Despite the diversity of microbiota in birds is similar to that of other animals, there is a lack of research on the gut microbial diversity of nondomesticated bird species. This study aims to address this gap in knowledge by analyzing the bacterial communities present in the gut of two important game bird species, the Ring-necked pheasant (Phasianus colchicus) and the Green pheasant (Phasianus versicolor) to understand the gut microbial diversity of these species. The gut microbiome of 10 individual pheasants from two different species was studied using pooled fecal samples. We used 16S rRNA gene sequencing on the Ion S5 XL System next-generation sequencing with Mothur and SILVA Database for taxonomic division. An average of 141 different operational taxonomic units were detected in the gut microbiome. Analysis of microbial classification revealed the presence of 191 genera belonging to 12 different phyla in both pheasants. Alpha diversity indices revealed that P. colchicus exhibited most prevalence firmicutes with bacillus species microbial community than P. versicolor. Alpha diversity indices indicated that P. colchicus had a more diverse community and P. versicolor had a greater diversity of evolutionary lineages, while both species had similar levels of species richness and sample inclusiveness. These findings may have implications for the health and well-being of pheasants, serving as a reference for their bacterial diversity. Additionally, they provide a baseline for future research and conservation efforts aimed at improving the health and well-being of these and possibly other avian species.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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