Low microbial diversity, yeast prevalence, and nematode-trapping fungal presence in fungal colonization and leaf microbiome of Serjania erecta.

Autor: de Freitas STF; Laboratory of Agricultural Microbiology, Instituto Federal Goiano - campus Rio Verde, Highway Sul Goiana, Km 01, Rio Verde, GO, 75901-970, Brazil., Silva FG; Laboratory of Plant Mineral Nutrition, Instituto Federal Goiano, campus Rio Verde, Rio Verde, Brazil., Bessa LA; Laboratory of Plant Mineral Nutrition, Instituto Federal Goiano, campus Rio Verde, Rio Verde, Brazil., de Souza UJB; Bioinformatics and Biotechnology Laboratory, Federal University of Tocantins, Campus of Gurupi, Gurupi, TO, 77410-570, Brazil., Augusto DSS; Laboratory of Agricultural Microbiology, Instituto Federal Goiano - campus Rio Verde, Highway Sul Goiana, Km 01, Rio Verde, GO, 75901-970, Brazil., de Faria GS; Laboratory of Agricultural Microbiology, Instituto Federal Goiano - campus Rio Verde, Highway Sul Goiana, Km 01, Rio Verde, GO, 75901-970, Brazil., Vitorino LC; Laboratory of Agricultural Microbiology, Instituto Federal Goiano - campus Rio Verde, Highway Sul Goiana, Km 01, Rio Verde, GO, 75901-970, Brazil. luciana.vitorino@ifgoiano.edu.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 04; Vol. 14 (1), pp. 15456. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1038/s41598-024-66161-3
Abstrakt: Medicinal plant microbiomes undergo selection due to secondary metabolite presence. Resident endophytic/epiphytic microorganisms directly influence plant's bioactive compound synthesis. Hypothesizing low microbial diversity in Serjania erecta leaves, we assessed leaf colonization by epiphytic and endophytic fungi. Given its traditional medicinal importance, we estimated diversity in the endophytic fungal microbiome. Analyses included scanning electron microscopy (SEM), isolation of cultivable species, and metagenomics. Epiphytic fungi interacted with S. erecta leaf tissues, horizontally transmitted via stomata/trichome bases, expressing traits for nematode trapping. Cultivable endophytic fungi, known for phytopathogenic habits, didn't induce dysbiosis symptoms. This study confirms low leaf microbiome diversity in S. erecta, with a tendency towards more fungal species, likely due to antibacterial secondary metabolite selection. The classification of Halicephalobus sp. sequence corroborated the presence of nematode eggs on the epidermal surface of S. erecta by SEM. In addition, we confirmed the presence of methanogenic archaea and a considerable number of methanotrophs of the genus Methylobacterium. The metagenomic study of endophytic fungi highlighted plant growth-promoting yeasts, mainly Malassezia, Leucosporidium, Meyerozyma, and Hannaella. Studying endophytic fungi and S. erecta microbiomes can elucidate their impact on beneficial bioactive compound production, on the other hand, it is possible that the bioactive compounds produced by this plant can recruit specific microorganisms, impacting the biological system.
(© 2024. The Author(s).)
Databáze: MEDLINE
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