State of art of micronuclei assay in exfoliative cytology as a clinical biomarker of genetic damage in oral carcinogenesis: A systematic review and meta-analysis.

Autor: Caponio VCA; Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy; ORALMED Research Group, Department of Dental Clinical Specialties, School of Dentistry, Complutense University, Madrid 28040, Spain., Silva FFE; Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain; ORALRES Group, Health Research Institute of Santiago de Compostela (FIDIS), Santiago de Compostela 15782, Spain. Electronic address: fabio.frana@rai.usc.es., Popolo F; Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy., Giugliano S; Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy., Spizzirri F; Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy., Lorenzo-Pouso AI; Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain., Padín-Iruegas ME; Human Anatomy and Embryology Area, Department of Functional Biology and Health Sciences, University of Vigo, Vigo 36310, Spain., Zhurakivska K; Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy., Muzio LL; Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy., López-Pintor RM; ORALMED Research Group, Department of Dental Clinical Specialties, School of Dentistry, Complutense University, Madrid 28040, Spain.
Jazyk: angličtina
Zdroj: Mutation research. Reviews in mutation research [Mutat Res Rev Mutat Res] 2024 Jul 02; Vol. 794, pp. 108508. Date of Electronic Publication: 2024 Jul 02.
DOI: 10.1016/j.mrrev.2024.108508
Abstrakt: Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, often preceded by oral potentially malignant disorders (OPMDs). Currently, no clinical biomarker exists to predict malignancy, necessitating OPMD follow-up. Habits and environmental factors, such as smoking, and alcohol consumption, influence OSCC onset. Increased micronuclei (MNs) formation has been observed in the development of OSCC. Non-invasive diagnostic tests like exfoliative cytology offer painless and regular monitoring options. This study evaluates the impact of tobacco, alcohol, and pesticide exposure on MNs occurrence in exfoliative cytology-collected oral mucosal cells, assessing their potential as non-invasive biomarker for OSCC development prediction and monitoring in high-risk patients. Despite results from this meta-analysis supporting the existence of a stepwise increase from controls to patients with OPMD to OSCC, the translation of these findings into clinical practice is limited due to intra- and inter-individual heterogeneity, as well as methodological variability in MNs quantification. Various factors contribute to this heterogeneity, including demographic variables, methodological variability of different laboratories, staining techniques, sample collection location, and patient characteristics. All these points were discussed to provide further insights and improve standardization for future studies.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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