TGFβ1 Regulates Cellular Composition of In Vitro Cardiac Perivascular Niche Based on Cardiospheres.

Autor: Goltseva YD; National Medical Research Centre of Cardiology named after academician E. I. Chazov, Moscow, Russia. eleagnusom@gmail.com., Dergilev KV; National Medical Research Centre of Cardiology named after academician E. I. Chazov, Moscow, Russia., Boldyreva MA; National Medical Research Centre of Cardiology named after academician E. I. Chazov, Moscow, Russia., Parfyonova EV; National Medical Research Centre of Cardiology named after academician E. I. Chazov, Moscow, Russia., Beloglazova IB; National Medical Research Centre of Cardiology named after academician E. I. Chazov, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2024 May; Vol. 177 (1), pp. 115-123. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1007/s10517-024-06142-8
Abstrakt: The cardiac perivascular niche is a cellular microenvironment of a blood vessel. The principles of niche regulation are still poorly understood. We studied the effect of TGFβ1 on cells forming the cardiac perivascular niche using 3D cell culture (cardiospheres). Cardiospheres contained progenitor (c-Kit), endothelial (CD31), and mural (αSMA) cells, basement membrane proteins (laminin) and extracellular matrix proteins (collagen I, fibronectin). TGFβ1 treatment decreased the length of CD31 + microvasculature, VE cadherin protein level, and proportion of NG2 + cells, and increased proportion of αSMA + cells and transgelin/SM22α protein level. We supposed that this effect is related to the stabilizing function of TGFβ1 on vascular cells: decreased endothelial cell proliferation, as shown for HUVEC, and activation of mural cell differentiation.
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: