Improved Preservation of Rat Small Intestine Transplantation Graft by Introduction of Mesenchymal Stem Cell-Secreted Fractions.

Autor:	Teratani T; Division of Translational Research, Jichi Medical University, Tochigi, Japan.; Department of Surgery, Jichi Medical University, Tochigi, Japan., Fujimoto Y; Transplantation Surgery, Nagoya University Hospital, Nagoya, Aichi, Japan., Sakuma Y; Department of Surgery, Jichi Medical University, Tochigi, Japan., Kasahara N; Division of Translational Research, Jichi Medical University, Tochigi, Japan.; Department of Surgery, Jichi Medical University, Tochigi, Japan., Maeda M; Division of Translational Research, Jichi Medical University, Tochigi, Japan., Miki A; Department of Surgery, Jichi Medical University, Tochigi, Japan., Lefor AK; Department of Surgery, Jichi Medical University, Tochigi, Japan., Sata N; Department of Surgery, Jichi Medical University, Tochigi, Japan., Kitayama J; Division of Translational Research, Jichi Medical University, Tochigi, Japan.; Department of Surgery, Jichi Medical University, Tochigi, Japan.
Jazyk:	angličtina
Zdroj:	Transplant international : official journal of the European Society for Organ Transplantation [Transpl Int] 2024 Jun 19; Vol. 37, pp. 11336. Date of Electronic Publication: 2024 Jun 19 (Print Publication: 2024).
DOI:	10.3389/ti.2024.11336
Abstrakt:	Segmental grafts from living donors have advantages over grafts from deceased donors when used for small intestine transplantation. However, storage time for small intestine grafts can be extremely short and optimal graft preservation conditions for short-term storage remain undetermined. Secreted factors from mesenchymal stem cells (MSCs) that allow direct activation of preserved small intestine grafts. Freshly excised Luc-Tg LEW rat tissues were incubated in preservation solutions containing MSC-conditioned medium (MSC-CM). Preserved Luc-Tg rat-derived grafts were then transplanted to wild-type recipients, after which survival, injury score, and tight junction protein expression were examined. Luminance for each graft was determined using in vivo imaging. The findings indicated that 30-100 and 3-10 kDa fractions of MSC-CM have superior activating effects for small intestine preservation. Expression of the tight-junction proteins claudin-3, and zonula occludens-1 preserved for 24 h in University of Wisconsin (UW) solution containing MSC-CM with 50-100 kDa, as shown by immunostaining, also indicated effectiveness. Reflecting the improved graft preservation, MSC-CM preloading of grafts increased survival rate from 0% to 87%. This is the first report of successful transplantation of small intestine grafts preserved for more than 24 h using a rodent model to evaluate graft preservation conditions that mimic clinical conditions. Competing Interests: Author MM was employed by the company Asteras. However, he is only involved in supporting small intestine transplants and has no relation to the company Astellas. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Teratani, Fujimoto, Sakuma, Kasahara, Maeda, Miki, Lefor, Sata and Kitayama.)
Databáze:	MEDLINE
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