Multicellular, IVT-derived, unmodified human transcriptome for nanopore-direct RNA analysis.

Autor:	McCormick CA; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA., Akeson S; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA., Tavakoli S; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA., Bloch D; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA., Klink IN; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA., Jain M; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA.; Department of Physics, Northeastern University, Boston, MA, 02115, USA., Rouhanifard SH; Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA.
Jazyk:	angličtina
Zdroj:	GigaByte (Hong Kong, China) [GigaByte] 2024 Jun 17; Vol. 2024, pp. gigabyte129. Date of Electronic Publication: 2024 Jun 17 (Print Publication: 2024).
DOI:	10.46471/gigabyte.129
Abstrakt:	Nanopore direct RNA sequencing (DRS) enables measurements of RNA modifications. Modification-free transcripts are a practical and targeted control for DRS, providing a baseline measurement for canonical nucleotides within a matched and biologically-derived sequence context. However, these controls can be challenging to generate and carry nanopore-specific nuances that can impact analyses. We produced DRS datasets using modification-free transcripts from in vitro transcription of cDNA from six immortalized human cell lines. We characterized variation across cell lines and demonstrated how these may be interpreted. These data will serve as a versatile control and resource to the community for RNA modification analyses of human transcripts. Competing Interests: The authors declare no competing interests. (© The Author(s) 2024.)
Databáze:	MEDLINE
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