The root-knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism.

Autor: Soulé S; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Huang K; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China., Mulet K; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Mejias J; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Bazin J; Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris Saclay - Evry, Université de Paris, Gif sur Yvette, France., Truong NM; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Kika JL; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Jaubert S; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Abad P; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Zhao J; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China., Favery B; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France., Quentin M; INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France.
Jazyk: angličtina
Zdroj: Molecular plant pathology [Mol Plant Pathol] 2024 Jul; Vol. 25 (7), pp. e13491.
DOI: 10.1111/mpp.13491
Abstrakt: Root-knot nematodes (RKNs) are microscopic parasitic worms able to infest the roots of thousands of plant species, causing massive crop yield losses worldwide. They evade the plant's immune system and manipulate plant cell physiology and metabolism to transform a few root cells into giant cells, which serve as feeding sites for the nematode. RKN parasitism is facilitated by the secretion in planta of effector molecules, mostly proteins that hijack host cellular processes. We describe here a conserved RKN-specific effector, effector 12 (EFF12), that is synthesized exclusively in the oesophageal glands of the nematode, and we demonstrate its function in parasitism. In the plant, MiEFF12 localizes to the endoplasmic reticulum (ER). A combination of RNA-sequencing analysis and immunity-suppression bioassays revealed the contribution of MiEFF12 to the modulation of host immunity. Yeast two-hybrid, split luciferase and co-immunoprecipitation approaches identified an essential component of the ER quality control system, the Solanum lycopersicum plant bap-like (PBL), and basic leucine zipper 60 (BZIP60) proteins as host targets of MiEFF12. Finally, silencing the PBL genes in Nicotiana benthamiana decreased susceptibility to Meloidogyne incognita infection. Our results suggest that EFF12 manipulates PBL function to modify plant immune responses to allow parasitism.
(© 2024 The Author(s). Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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