| Autor: |
Alves MDR; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com., Nascimento RP; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com., da Fonseca Machado AP; Universidade Federal da Grande Dourados, Faculdade de Engenharia, Rod. Dourados-Itahum Km 12, C.P.: 79804-970 - Dourados, Mato Grosso do Sul, Brasil., Dos Santos P; Rubian xtratos LTDA, Rua do Café, 375 Vila Valle, 13174-000, Sumaré, São Paulo, Brazil., Aledo E; Rubian xtratos LTDA, Rua do Café, 375 Vila Valle, 13174-000, Sumaré, São Paulo, Brazil., Morandi Vuolo M; Rubian xtratos LTDA, Rua do Café, 375 Vila Valle, 13174-000, Sumaré, São Paulo, Brazil., Cavalheiro CO; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com., Giaculi VO; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com., Berilli P; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com., Dos Santos NM; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com., Marostica Junior MR; Universidade Estadual de Campinas, School of Food Engineering, Department of Food Science and Nutrition, Laboratory of Nutrition and Metabolism, 80 Rua Monteiro Lobato, 13083-862 Campinas, São Paulo, Brazil. mariana.roalves@gmail.com. |
| Abstrakt: |
The rates of overweight and obesity around the world have increased in past years. The body's adipose tissue stimulates the antioxidant and oxidation imbalance capacity at the cellular level. This scenario favors an inflammatory low-grade systemic condition starting with insulin resistance, which in turn may involve diabetes mellitus type 2 and cognitive decline afterward. Neurological diseases have been correlated to senile age diseases over time. This scenario calls for a change in the incidence of obesity in the younger generation. An unhealthy dietary consumption together with sedentary habits might lead to poor gut absorption of nutrients. Several plants and foods have bioactive compounds that can reduce or inhibit radical scavengers, reactive oxygen species, and metal ion complexes that threaten the cerebral defense system. The bitter acids from hops ( Humulus lupulus L.) have been demonstrated to have promising effects on lipid and carbohydrate metabolism improvement, reducing inflammatory responses through alpha acids, beta acids, and analogs action. Therefore, the current study aimed to investigate the bioactivity of hop bitter acids in obese and lean mice. For that, a dry hop extract (DHE) was obtained by applying carbon dioxide as the fluid of supercritical extraction. Afterward, seventy-eight male mice of the C57BL/6J strain were weighed and randomly distributed into six groups of 13 animals each according to the diet offered: (NO) normolipidic diet, (NO1) normolipidic diet containing 0.35% alpha acids, (NO2) normolipidic diet containing 3.5% alpha acids, (HP) hyperlipidic diet, (HP1) hyperlipidic diet containing 0.35% alpha acids, and (HP2) hyperlipidic diet containing 3.5% alpha acids. After applying the glycemic tolerance and insulin tolerance tests, a better stabilization of glycemia levels and weight gain among those animals fed with DHE (NO2 and HP2) were observed in comparison to the obese control group (HP) ( p < 0.05). There was also an amelioration of antioxidant capacity observed by checking the enzymatic profile by SOD and an apparent mitigation of brain degeneration by checking GSK3β and p-IRS1 proteins expression ( p < 0.05). The y-maze cognitive test applied to highlight possible obesity-harmful animal brains did not indicate a statistical difference between the groups. Although the weekly dietary intake between the obese HP2 group (33.32 ± 4.11, p < 0.05) and control HP (42.3 ± 5.88, p < 0.05) was different. The bioactive compounds present in DHE have demonstrated relevant effects on glycemic control, insulin signaling, and the consequent modulatory action of the obesity-related markers with the brain's inflammatory progression. |
