Examining effects of a novel estrogenic perfluoro-alcohol, 1H,1H,8H,8H-Perfluorooctane-1,8-diol (FC8-diol), using the fathead minnow EcoToxChip.

Autor: Santana Rodriguez KJ; Great Lakes Toxicology and Ecology Division, Oak Ridge Institute for Science and Education, US Environmental Protection Agency, Duluth, Minnesota., Villeneuve DL; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Cavallin JE; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Blackwell BR; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Hoang J; Great Lakes Toxicology and Ecology Division, Oak Ridge Institute for Science and Education, US Environmental Protection Agency, Duluth, Minnesota., Hofer RN; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Jensen KM; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Kahl MD; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Kutsi RN; Great Lakes Toxicology and Ecology Division, Oak Ridge Institute for Science and Education, US Environmental Protection Agency, Duluth, Minnesota., Stacy E; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota., Morshead ML; Great Lakes Toxicology and Ecology Division, Oak Ridge Institute for Science and Education, US Environmental Protection Agency, Duluth, Minnesota., Ankley GT; Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency, Duluth, Minnesota.
Jazyk: angličtina
Zdroj: Environmental toxicology and chemistry [Environ Toxicol Chem] 2024 Jul 03. Date of Electronic Publication: 2024 Jul 03.
DOI: 10.1002/etc.5937
Abstrakt: In a previous in vivo study, adult male fathead minnows (Pimephales promelas) were exposed via water for 4 days to 1H,1H,8H,8H-perfluorooctane-1,8-diol (FC8-diol). The present study expands on the evaluation of molecular responses to this perfluoro-alcohol by analyzing 26 male fathead minnow liver RNA samples from that study (five from each test concentration: 0, 0.018, 0.051, 0.171, and 0.463 mg FC8-diol/L) using fathead minnow EcoToxChips Ver. 1.0. EcoToxChips are a quantitative polymerase chain reaction array that allows for simultaneous measurement of >375 species-specific genes of toxicological interest. Data were analyzed with the online tool EcoToxXplorer. Among the genes analyzed, 62 and 96 were significantly up- and downregulated, respectively, by one or more FC8-diol treatments. Gene expression results from the previous study were validated, showing an upregulation of vitellogenin mRNA (vtg) and downregulation of insulin-like growth factor 1 mRNA (igf1). Additional genes related to estrogen receptor activation including esr2a (estrogen receptor 2a) and esrrb (estrogen related receptor beta) were also affected, providing further confirmation of the estrogenic nature of FC8-diol. Furthermore, genes involved in biological pathways related to lipid and carbohydrate metabolism, innate immune response, endocrine reproduction, and endocrine thyroid were significantly affected. These results both add confidence in the use of the EcoToxChip tool for inferring chemical mode(s) of action and provide further insights into the possible biological effects of FC8-diol. Environ Toxicol Chem 2024;00:1-9. © 2024 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
(© 2024 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)
Databáze: MEDLINE
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