Metabarcoding expands knowledge on diversity and ecology of rare actinobacteria in the Brazilian Cerrado.

Autor: Bandeira L; Ecology and Natural Resources, Federal University of Ceará, Fortaleza, Brazil. leonardobandeira@alu.ufc.br.; Graduate Course of Ecology and Natural Resources, Department of Biology, Campus of Pici, Federal University of Ceará, Fortaleza, 60440-900, Brazil. leonardobandeira@alu.ufc.br., Faria C; Biomolecular Sciences, University of East Anglia, Norwich, UK., Cavalcante F; Ecology and Natural Resources, Federal University of Ceará, Fortaleza, Brazil., Mesquita A; Biotechnology of Natural Resources, Federal University of Ceará, Fortaleza, Brazil., Martins C; Graduate Course of Ecology and Natural Resources, Department of Biology, Campus of Pici, Federal University of Ceará, Fortaleza, 60440-900, Brazil., Martins S; Graduate Course of Ecology and Natural Resources, Department of Biology, Campus of Pici, Federal University of Ceará, Fortaleza, 60440-900, Brazil.
Jazyk: angličtina
Zdroj: Folia microbiologica [Folia Microbiol (Praha)] 2024 Jul 03. Date of Electronic Publication: 2024 Jul 03.
DOI: 10.1007/s12223-024-01184-x
Abstrakt: Rare and unknown actinobacteria from unexplored environments have the potential to produce new bioactive molecules. This study aimed to use 16 s rRNA metabarcoding to determine the composition of the actinobacterial community, particularly focusing on rare and undescribed species, in a nature reserve within the Brazilian Cerrado called Sete Cidades National Park. Since this is an inaccessible area without due legal authorization, it is understudied, and, therefore, its diversity and biotechnological potential are not yet fully understood, and it may harbor species with groundbreaking genetic potential. In total, 543 operational taxonomic units (OTUs) across 14 phyla were detected, with Actinobacteria (41.2%), Proteobacteria (26.5%), and Acidobacteria (14.3%) being the most abundant. Within Actinobacteria, 107 OTUs were found, primarily from the families Mycobacteriaceae, Pseudonocardiaceae, and Streptomycetaceae. Mycobacterium and Streptomyces were the predominant genera across all samples. Seventeen rare OTUs with relative abundance < 0.1% were identified, with 82.3% found in only one sample yet 25.5% detected in all units. Notable rare and transient genera included Salinibacterium, Nocardia, Actinomycetospora_01, Saccharopolyspora, Sporichthya, and Nonomuraea. The high diversity and distribution of Actinobacteria OTUs indicate the area's potential for discovering new rare species. Intensified prospection on underexplored environments and characterization of their actinobacterial diversity could lead to the discovery of new species capable of generating innovative natural products.
(© 2024. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.)
Databáze: MEDLINE
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