Innate and adaptive immune response of Rainbow trout (Oncorhynchus mykiss) naturally infected with Yersinia ruckeri.

Autor: Soto-Dávila M; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada; Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, C1A 4P3, Canada., Rodríguez-Cornejo T; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada; Faculty of Veterinary Medicine and Zootechnics, Universidad Peruana Cayetano Heredia, Lima, Peru., Benito VW; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Rodríguez-Ramos T; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Mahoney G; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Supinski R; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Heath G; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Dang X; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Valle FM; CITEacuícola Pesquero Ahuashiyacu, Instituto Tecnológico de la Producción, La Banda de Shilcayo CP, 22200, San Martín, Peru., Hurtado C; Faculty of Veterinary Medicine and Zootechnics, Universidad Peruana Cayetano Heredia, Lima, Peru., Llanco LA; School of Human Medicine, Universidad Privada San Juan Bautista, Apartado, Chincha, 15067, Peru., Serrano-Martinez E; Faculty of Veterinary Medicine and Zootechnics, Universidad Peruana Cayetano Heredia, Lima, Peru., Dixon B; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada. Electronic address: bdixon@uwaterloo.ca.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Aug; Vol. 151, pp. 109742. Date of Electronic Publication: 2024 Jul 02.
DOI: 10.1016/j.fsi.2024.109742
Abstrakt: Rainbow trout is an important fish species for Peruvian artisanal aquaculture, comprising over 60 % of the total aquaculture production. However, their industry has been highly affected by several bacterial agents such as Yersinia ruckeri. This pathogen is the causative agent of Enteric Redmouth Disease, and causes high mortality in fingerlings and chronic infection in adult rainbow trout. To date, the immune response of rainbow trout against Y. ruckeri has been well studied in laboratory-controlled infection studies (i.e. intraperitoneal infection, bath immersion), however, the immune response during natural infection has not been explored. To address this, in this study, 35 clinically healthy O. mykiss without evidence of lesions or changes in behavior and 32 rainbow trout naturally infected by Y. ruckeri, were collected from semi-intensive fish farms located in the Central Highlands of Peru. To evaluate the effect on the immune response, RT-qPCR, western blotting, and ELISA were conducted using head kidney, spleen, and skin tissues to evaluate the relative gene expression and protein levels. Our results show a significant increase in the expression of the pro-inflammatory cytokines il1b, tnfa, and il6, as well as ifng in all three tissues, as well as increases in IL-1β and IFN-γ protein levels. The endogenous pathway of antigen presentation showed to play a key role in defense against Y. ruckeri, due to the upregulation of mhc-I, tapasin, and b2m transcripts, and the significant increase of Tapasin protein levels in infected rainbow trout. None of the genes associated with the exogenous pathway of antigen presentation showed a significant increase in infected fish, suggesting that this pathway is not involved in the response against this intracellular pathogen. Finally, the transcripts of immunoglobulins IgM and IgT did not show a modulation, nor were the protein levels evaluated in this study.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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