Effect of extraction method on the calcium binding capacity of faba bean globulin fractions at various pH.

Autor: Amat T; Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, UMR PAM 1517, 21000 Dijon, France., Assifaoui A; Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, UMR PAM 1517, 21000 Dijon, France., Buczkowski J; Nestlé Research, Nestlé Institute of Food Sciences, Department of Nutrient Technology, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland., Silva JVC; Nestlé Research, Nestlé Institute of Food Sciences, Department of Nutrient Technology, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland., Schmitt C; Nestlé Research, Nestlé Institute of Food Sciences, Department of Nutrient Technology, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland., Saurel R; Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, UMR PAM 1517, 21000 Dijon, France. Electronic address: remi.saurel@agrosupdijon.fr.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Nov 15; Vol. 458, pp. 140176. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1016/j.foodchem.2024.140176
Abstrakt: Faba bean ingredients are rich in proteins and good sources of calcium (Ca), although containing phytic acid (PA) molecules. PA, a polyphosphate compound, can affect the bioavailability of minerals/proteins through complex formation. This study evaluates the impact of two extraction processes, Alkaline Extraction-IsoElectric Precipitation (AE-IEP) and Sequential Extraction (SE), on the ability of faba bean globulin systems to bind added calcium ions. Increasing concentrations of CaCl 2 were introduced into 2.5% (w/v) protein dispersions at pHs 4.5, 5.5, 6.5, and 7.5, and free Ca monitored. Near the isoelectric point of globulin (pH ∼ 4-5), Ca binding capacity was found to be low. At higher pHs, significant Ca chelation occurred, initially attributed to free PA binding sites, resulting in the formation of insoluble complexes and subsequent protein precipitation. The AE-IEP globulin fraction exhibited a higher Ca binding capacity than the SE globulin, attributed to its higher PA and lower initial Ca concentrations.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: