A novel polydiacetylene-functionalized fibrinogen paper-based biosensor for on-spot and rapid detection of Staphylococcus aureus.

Autor: Albashir D; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China., Lu H; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China., Gouda M; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Nutrition and Food Science, National Research Centre, Dokki, Giza 12622, Egypt., Acharya DR; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China., Danhassan UA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China., Bakur A; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Food Sciences and Technology, University of Kordofan, El Obeid, Sudan., Shi Y; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: shiying0520@zju.edu.cn., Chen Q; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: chenqh@zju.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Nov 15; Vol. 458, pp. 140291. Date of Electronic Publication: 2024 Jul 01.
DOI: 10.1016/j.foodchem.2024.140291
Abstrakt: Staphylococcus aureus contamination continues to be a harmful foodborne pathogen threatening of human health, and there is a growing need for rapid detection technologies. This study proposed a novel paper biosensor based on a polydiacetylene (PDA) polymer functionalized fibrinogen (Fg) for the detection of S. aureus in food sources. The fluorophore was developed based on the high binding ability of fibrinogen-binding proteins on the surface of S. aureus. This binding caused twisting in the PDA backbone, leading to changes in chromatic and fluorescent. The detection limit of this method was 50.1 CFU/mL for S. aureus-contaminated foodstuffs and 65.0 CFU/mL for the pure S. aureus culture, and the novelty came from its rapidity and selectivity for S. aureus compared to other foodborne bacteria. In summary, the present work provides a rapid detection method for S. aureus detection, which will help in addressing food safety-related issues.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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