| Autor: |
Starke N; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Challa NVD; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Yuan H; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Chen S; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Duncan MR; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Cabrera Ranaldi EDLRM; Miami Project to Cure Paralysis.; Department of Neurological Surgery, and., de Rivero Vaccari JP; Miami Project to Cure Paralysis.; Department of Neurological Surgery, and., Schott A; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Aguilar AC; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Lee YS; Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, Miami, Florida., Khan A; Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, Miami, Florida., Duara J; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Tan A; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Benny M; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Schmidt AF; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Young K; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Bancalari E; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Claure N; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital., Wu S; Division of Neonatology, Department of Pediatrics, Batchelor Children's Research Institute, Holtz Children's Hospital. |
| Abstrakt: |
Bronchopulmonary dysplasia (BPD) and neurodevelopmental impairment are among the most common morbidities affecting preterm infants. Although BPD is a predictor of poor neurodevelopmental outcomes, it is currently uncertain how BPD contributes to brain injury in preterm infants. Extracellular vesicles (EVs) are involved in interorgan communication in diverse pathological processes. ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) is pivotal in inflammasome assembly and activation of inflammatory response. We assessed expression profiles of the alveolar macrophage (AM) markers CD11b, CD11c, and CD206 as well as ASC in EVs isolated from the plasma of preterm infants at risk for BPD at 1 week of age. We found that infants on higher fraction of inspired oxygen therapy (HO 2 ⩾30%) had increased concentrations of AM-derived EV-ASC compared with infants on lower fraction of inspired oxygen (LO 2 <30%). To assess the function of these EVs, we performed adoptive transfer experiments by injecting them into the circulation of newborn mice. We discovered that mice that received EVs from infants on HO 2 had increased lung inflammation, decreased alveolarization, and disrupted vascular development, the hallmarks of BPD. Importantly, these EVs crossed the blood-brain barrier, and the EVs from infants on HO 2 caused inflammation, reduced cell survival, and increased cell death, with features of pyroptosis and necroptosis in the hippocampus. These results highlight a novel role for AM-derived EV-ASC in mediating the lung-to-brain cross-talk that is critical in the pathogenesis of BPD and brain injury and identify potential novel targets for preventing and treating BPD and brain injury in preterm infants. |
