Fluorogenic Monitoring of α-Amylase in Human Urine for Straightforward Diagnosis of Pancreatic Diseases.

Autor: Choi H; Department of Chemistry, Daegu University, Gyeongsan, 38453, Republic of Korea., Kim S; Department of Chemistry, Daegu University, Gyeongsan, 38453, Republic of Korea., Park T; Department of Chemistry, Daegu University, Gyeongsan, 38453, Republic of Korea., Lee SH; Department of Chemistry, Daegu University, Gyeongsan, 38453, Republic of Korea.
Jazyk: angličtina
Zdroj: Chemistry, an Asian journal [Chem Asian J] 2024 Oct 01; Vol. 19 (19), pp. e202400505. Date of Electronic Publication: 2024 Aug 21.
DOI: 10.1002/asia.202400505
Abstrakt: In this study, we developed a sensitive method for monitoring α-amylase using a fluorogenic approach based on the host-guest complexation between an amphiphilic pyrenyl derivative (1) and γ-cyclodextrins (γ-CDs). The compound 1 self-assembles into nanofibrils in aqueous solutions. Upon the introduction of γ-CD, compound 1 forms an inclusion complex with it. This complex then participates in the formation of a 2 : 2 complex with another complex, leading to strong excimer fluorescence. Upon interaction with α-amylase, γ-CD undergoes hydrolysis, leading to the regeneration of nanofibrils, which is accompanied by a decrease in excimer fluorescence and an increase in monomeric fluorescence. This ratiometric fluorescence color change enables the sensitive detection of low levels of α-amylase in human urine, offering a practical approach for early screening of pancreatic-related diseases.
(© 2024 Wiley-VCH GmbH.)
Databáze: MEDLINE
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