Multiplexed in situ hybridization reveals distinct lineage identities for major and minor vein initiation during maize leaf development.

Autor:	Perico C; Department of Biology, University of Oxford, Oxford OX1 3RB, United Kingdom., Zaidem M; Department of Biology, University of Oxford, Oxford OX1 3RB, United Kingdom., Sedelnikova O; Department of Biology, University of Oxford, Oxford OX1 3RB, United Kingdom., Bhattacharya S; Resolve BioSciences GmbH, 40789 Monheim am Rhein, Germany., Korfhage C; Resolve BioSciences GmbH, 40789 Monheim am Rhein, Germany., Langdale JA; Department of Biology, University of Oxford, Oxford OX1 3RB, United Kingdom.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jul 09; Vol. 121 (28), pp. e2402514121. Date of Electronic Publication: 2024 Jul 03.
DOI:	10.1073/pnas.2402514121
Abstrakt:	Leaves of flowering plants are characterized by diverse venation patterns. Patterning begins with the selection of vein-forming procambial initial cells from within the ground meristem of a developing leaf, a process which is considered to be auxin-dependent, and continues until veins are anatomically differentiated with functional xylem and phloem. At present, the mechanisms responsible for leaf venation patterning are primarily characterized in the model eudicot Arabidopsis thaliana which displays a reticulate venation network. However, evidence suggests that vein development may proceed via a different mechanism in monocot leaves where venation patterning is parallel. Here, we employed Molecular Cartography, a multiplexed in situ hybridization technique, to analyze the spatiotemporal localization of a subset of auxin-related genes and candidate regulators of vein patterning in maize leaves. We show how different combinations of auxin influx and efflux transporters are recruited during leaf and vein specification and how major and minor vein ranks develop with distinct identities. The localization of the procambial marker PIN1a and the spatial arrangement of procambial initial cells that give rise to major and minor vein ranks further suggests that vein spacing is prepatterned across the medio-lateral leaf axis prior to accumulation of the PIN1a auxin transporter. In contrast, patterning in the adaxial-abaxial axis occurs progressively, with markers of xylem and phloem gradually becoming polarized as differentiation proceeds. Collectively, our data suggest that both lineage- and position-based mechanisms may underpin vein patterning in maize leaves. Competing Interests: Competing interests statement:S.B. and C.K. are employees of Resolve Biosciences whose Molecular Cartography technology was used for multiplexed in situ hybridization.
Databáze:	MEDLINE
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