A comprehensive Study of Juglone's Effect on Polyphenol Oxidase in Cucumber: In Vitro Experiments and Computational Docking and Dynamics Insights.

Autor: Kocaçalışkan İ; Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, İstanbul, Türkiye., Korkut ŞV; Department of Molecular Biology and Genetics, Faculty of Science and Letter, Yıldız Technical University, İstanbul, Türkiye., Aktaş E; Department of Molecular Biology and Genetics, Faculty of Science and Letter, Yıldız Technical University, İstanbul, Türkiye., Yalçın M; Institute of Science and Technology, Yıldız Technical University, İstanbul, Türkiye., Özgentürk NÖ; Department of Molecular Biology and Genetics, Faculty of Science and Letter, Yıldız Technical University, İstanbul, Türkiye.
Jazyk: angličtina
Zdroj: Physiologia plantarum [Physiol Plant] 2024 Jul-Aug; Vol. 176 (4), pp. e14420.
DOI: 10.1111/ppl.14420
Abstrakt: This study explores the impact of juglone on cucumber (Cucumis sativus cv. Beith Alpha), scrutinizing its effects on seed germination, growth, and the polyphenol oxidase (PPO) enzyme's activity and gene expression. Employing concentrations ranging from 0.01 to 0.5 mM, we found juglone's effects to be concentration-dependent. At lower concentrations (0.01 and 0.1 mM), juglone promoted root and shoot growth along with germination, whereas higher concentrations (0.25 and 0.5 mM) exerted inhibitory effects, delineating a threshold for its allelopathic influence. Notably, PPO activity surged, especially at 0.5 mM in roots, hinting at oxidative stress involvement. Real-time PCR unveiled that juglone modulates PPO gene expression in cotyledons, peaking at 0.1 mM and diminishing at elevated levels. Correlation analyses elucidated a positive link between juglone-induced root growth and cotyledon PPO gene expression but a negative correlation with heightened root enzyme activity. Additionally, germination percentage inversely correlated with root PPO activity, while PPO activities positively associated with dopa and catechol substrates in both roots and cotyledons. Molecular docking studies revealed juglone's selective interactions with PPO's B chain, suggesting regulatory impacts. Protein interaction assessments highlighted juglone's influence on amino acid metabolism, and molecular dynamics indicated juglone's stronger, more stable binding to PPO, inferring potential alterations in enzyme function and stability. Conclusively, our findings elucidate juglone's dose-dependent physiological and biochemical shifts in cucumber plants, offering insights into its role in plant growth, stress response, and metabolic modulation.
(© 2024 The Author(s). Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.)
Databáze: MEDLINE
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