Investigation on biofilm composition and virulence traits of S. pseudintermedius isolated from infected and colonized dogs.

Autor: Teixeira IM; Laboratório de Biologia de Anaeróbios, Depto. Microbiologia Médica, IMPPG, UFRJ, Rio de Janeiro, Brazil.; Laboratório de Cocos Gram Positivos, Depto. de Microbiologia e Parasitologia, UFF, Rio de Janeiro, Brazil., de Moraes Assumpção Y; Laboratório de Cocos Gram Positivos, Depto. de Microbiologia e Parasitologia, UFF, Rio de Janeiro, Brazil., Paletta ACC; Laboratório de Cocos Gram Positivos, Depto. de Microbiologia e Parasitologia, UFF, Rio de Janeiro, Brazil., Antunes M; Laboratório de Biologia de Anaeróbios, Depto. Microbiologia Médica, IMPPG, UFRJ, Rio de Janeiro, Brazil., da Silva IT; Laboratório de Cocos Gram Positivos, Depto. de Microbiologia e Parasitologia, UFF, Rio de Janeiro, Brazil., Jaeger LH; Laboratório de Células-Tronco e Parasitologia Molecular, Depto. de Ciências Farmacêuticas, UFJF, Rio de Janeiro, Brazil., Ferreira RF; Curso de Medicina Veterinária da Universidade de Vassouras - Diagnóstico em medicina veterinária - Vassouras, Rio de Janeiro, Brazil., de Oliveira Ferreira E; Laboratório de Biologia de Anaeróbios, Depto. Microbiologia Médica, IMPPG, UFRJ, Rio de Janeiro, Brazil., de Araújo Penna B; Laboratório de Cocos Gram Positivos, Depto. de Microbiologia e Parasitologia, UFF, Rio de Janeiro, Brazil. bpenna@id.uff.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Sep; Vol. 55 (3), pp. 2923-2936. Date of Electronic Publication: 2024 Jul 03.
DOI: 10.1007/s42770-024-01405-y
Abstrakt: Staphylococcus pseudintermedius, which is part of the skin microbiome of dogs, causes a variety of opportunistic infections. These infections may become more difficult to treat due to the formation of biofilm. The capacity of S. pseudintermedius to form biofilm, as well as the associated genes, has not been elucidated. This study evaluated the production and composition of S. pseudintermedius biofilm. Samples were collected from both infected dogs and asymptomatic dogs. Isolates were identified using mass spectrometry and Multiplex-PCR. Biofilm production and composition were assessed using a quantitative microtiter plate assay. The presence of ica operon genes and sps genes was investigated using conventional PCR. The investigation of Agr type and virulence genes was conducted in silico on 24 sequenced samples. All strains could produce strong biofilms, with most of the isolates presenting a polysaccharide biofilm. 63.6% of the isolates carried the complete ica operon (ADBC). All samples showed the presence of the genes spsK, spsA, and spsL, while the distribution of other genes varied. Agr type III was the most prevalent (52.2%). All sequenced samples carried the cytotoxins hlb, luk-S, luk-F, as well as the exfoliative toxins siet and se_int. No isolate displayed other exfoliative toxins. Only LB1733 presented a set of different enterotoxins (sea, seb, sec_canine, seh, sek, sel, and seq). Our findings suggest that S. pseudintermedius is a strong producer of biofilm and carries virulence genes.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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