Antibacterial and Antibiofilm Activity of Croton urticifolius Lam. Essential Oil Via Membrane Disruption.

Autor: Malveira EA; Laboratório Integrado de Biomoléculas, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., Nunes AKA; Faculdade de Ciências Exatas E Naturais, Universidade Do Estado Do Rio Grande Do Norte, Mossoró, RN, Brazil., Andrade AL; Laboratório Integrado de Biomoléculas, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., Melo GLC; Laboratório Integrado de Biomoléculas, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., da Silva WMB; Centro de Ciência E Tecnologia, Universidade Estadual Do Ceará, Fortaleza, CE, Brasil., de Morais SM; Centro de Ciência E Tecnologia, Universidade Estadual Do Ceará, Fortaleza, CE, Brasil., Dos Santos HS; Laboratório Integrado de Biomoléculas, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.; Centro de Ciências Exatas E Tecnologia, Universidade Estadual Do Vale Do Acaraú, Sobral, CE, Brazil., de Lima LB; Faculdade de Ciências Exatas E Naturais, Universidade Do Estado Do Rio Grande Do Norte, Mossoró, RN, Brazil., de Albuquerque CC; Faculdade de Ciências Exatas E Naturais, Universidade Do Estado Do Rio Grande Do Norte, Mossoró, RN, Brazil., do Nascimento Souza DN; Faculdade de Ciências Exatas E Naturais, Universidade Do Estado Do Rio Grande Do Norte, Mossoró, RN, Brazil., Teixeira EH; Laboratório Integrado de Biomoléculas, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., de Vasconcelos MA; Faculdade de Educação de Itapipoca, Universidade Estadual Do Ceará, Itapipoca, CE, Brazil. mayronvasconcelos@gmail.com.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2024 Jul 02; Vol. 81 (8), pp. 256. Date of Electronic Publication: 2024 Jul 02.
DOI: 10.1007/s00284-024-03779-6
Abstrakt: Antimicrobial resistance is a global health issue, in which microorganisms develop resistance to antimicrobial drugs, making infections more difficult to treat. This threatens the effectiveness of standard medical treatments and necessitates the urgent development of new strategies to combat resistant microbes. Studies have increasingly explored natural sources of new antimicrobial agents that harness the rich diversity of compounds found in plant species. This pursuit holds promise for the discovery of novel treatments for combating antimicrobial resistance. In this context, the chemical composition, antibacterial, and antibiofilm activities of the essential oil from Croton urticifolius Lam. leaves (CuEO) were evaluated. CuEO was extracted via hydrodistillation, and its chemical constituents were identified via gas chromatography-mass spectrometry (GC/MS). The antibacterial activity of CuEO was evaluated in a 96-well plate via the microdilution method, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined. The effect of CuEO on biofilm formation was assessed by quantifying the biomass using crystal violet staining and viable cell counting. In addition, alterations in the cellular morphology of biofilms treated with CuEO were examined using scanning electron microscopy (SEM) and laser confocal microscopy. GC/MS analysis identified 26 compounds, with elemicine (39.72%); eucalyptol (19.03%), E-caryophyllene (5.36%), and methyleugenol (4.12%) as the major compounds. In terms of antibacterial activity, CuEO showed bacteriostatic effects against Staphylococcus aureus ATCC 700698, S. aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, and Escherichia coli ATCC 11303, and bactericidal activity against S. aureus ATCC 700698. In addition, CuEO significantly inhibited bacterial biofilm formation. Microscopic analysis showed that CuEO damaged the bacterial membrane by leaching out the cytoplasmic content. Therefore, the results of this study show that the essential oil of C. urticifolius may be a promising natural alternative for preventing infections caused by bacterial biofilms. This study is the first to report the antibiofilm activity of C. urticifolius essential oil.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: