Biotransformation of hydrocortisone succinate with whole cell cultures of Monascus purpureus and Cunninghamella echinulata.

Autor: Rahman Khan A; Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan., Aziz Z; Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan., Iqbal A; Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan., Sheema; Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan., Rashid Khan A; Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan., Zafar S; Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address: salmanzafar@uop.edu.pk.
Jazyk: angličtina
Zdroj: Steroids [Steroids] 2024 Sep; Vol. 209, pp. 109466. Date of Electronic Publication: 2024 Jun 30.
DOI: 10.1016/j.steroids.2024.109466
Abstrakt: Hydrocortisone succinate (1) is a synthetic anti-inflammatory drug and key intermediate in the synthesis of other steroidal drugs. This work is based on the fungal biotransformation of 1, using Monascus purpureus and Cunninghamella echinulata strains. Comopound 1 was transformed into four metabolites, identified as hydrocortisone (2), 11β-hydroxyandrost-4-en-3,17-dione (3), Δ 1 -cortienic acid (4), and hydrocortisone-17-succinate (5), obtained through side chain cleavage, hydrolysis, dehydrogenation, and oxidation reactions. These compounds have previously been synthesized either chemically or enzymatically from different precursors. Though this is not the first report on the biotransformation of 1, but it obviously is a first, where the biotransformed products of compound 1 have been characterized structurally with the help of modern spectroscopic techniques. It is noteworthy that these products have already shown biological potential, however a more thorough investigation of the anti-inflammatory properties of these metabolites would be of high value. These results not only emphasize upon the immense potential of biotransformation in catalysis of reactions, otherwise not-achievable chemically, but also holds promise for the development of novel anti-inflammatory compounds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: