The effects of ACE inhibitor Enalapril on Mytilus galloprovincialis: Insights into morphological and functional responses.

Autor: Filice M; Dept of Biology, Ecology and Earth Science, University of Calabria, Rende (CS), Italy. Electronic address: mariacristina.filice@unical.it., Caferro A; Dept of Biology, Ecology and Earth Science, University of Calabria, Rende (CS), Italy., Amelio D; Dept of Biology, Ecology and Earth Science, University of Calabria, Rende (CS), Italy., Impellitteri F; Dept of Veterinary Sciences, University of Messina, Messina, Italy., Iovine MA; Dept of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Naples, Italy., Porretti M; Dept of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy., Faggio C; Dept of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Naples, Italy; Dept of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy., Gattuso A; Dept of Biology, Ecology and Earth Science, University of Calabria, Rende (CS), Italy. Electronic address: alfonsina.gattuso@unical.it., Cerra MC; Dept of Biology, Ecology and Earth Science, University of Calabria, Rende (CS), Italy., Imbrogno S; Dept of Biology, Ecology and Earth Science, University of Calabria, Rende (CS), Italy.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2024 Aug; Vol. 273, pp. 107014. Date of Electronic Publication: 2024 Jun 27.
DOI: 10.1016/j.aquatox.2024.107014
Abstrakt: In the last decades, pharmaceuticals have emerged as a new class of environmental contaminants. Antihypertensives, including angiotensin-converting enzyme (ACE) inhibitors, are of special concern due to their increased consumption over the past years. However, the available data on their putative effects on the health of aquatic animals, as well as the possible interaction with biological systems are still poorly understood. This study analysed whether and to which extent the exposure to Enalapril, an ACE inhibitor commonly used for treating hypertension and heart failure, may induce morpho-functional alterations in the mussel Mytilus galloprovincialis, a sentinel organism of water pollution. By mainly focusing on the digestive gland (DG), a target tissue used for analysing the effects of xenobiotics in mussels, the effects of 10-days exposure to 0.6 ng/L (E1) and 600 ng/L (E2) of Enalapril were investigated in terms of cell viability and volume regulation, morphology, oxidative stress, and stress protein expression and localization. Results indicated that exposure to Enalapril compromised the capacity of DG cells from the E2 group to regulate volume by limiting the ability to return to the original volume after hypoosmotic stress. This occurred without significant effects on DG cell viability. Enalapril unaffected also haemocytes viability, although an increased infiltration of haemocytes was histologically observed in DG from both groups, suggestive of an immune response. No changes were observed in the two experimental groups on expression and tissue localization of heat shock proteins 70 (HSPs70) and HSP90, and on the levels of oxidative biomarkers. Our results showed that, in M. galloprovincialis the exposure to Enalapril did not influence the oxidative status, as well as the expression and localization of stress-related proteins, while it activated an immune response and compromised the cell ability to face osmotic changes, with potential consequences on animal performance.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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