Controlling the Subcellular Localization of Signaling Proteins Using Chemically Induced Dimerization and Optogenetics.
| Autor: | Beshay M; Department of Biological Sciences, University of the Sciences, Philadelphia, PA, USA., Deng Y; Department of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA., Janetopoulos C; Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. Cjaneto1@jh.edu.; Total Experience Learning, Albright College, Reading, PA, USA. Cjaneto1@jh.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2814, pp. 107-118. |
| DOI: | 10.1007/978-1-0716-3894-1_8 |
| Abstrakt: | A given protein can perform numerous roles in a cell with its participation in protein complexes and distinct localization within the cell playing a critical role in its diverse functions. Thus, the ability to artificially dimerize proteins and recruit proteins to specific locations in a cell has become a powerful tool for the investigation of protein function and the understanding of cell biology. Here, we discuss two systems that have been used to activate signal transduction pathways, a chemically inducible dimerization (CID) and a light-inducible (LI) system to control signaling and cytoskeletal regulation in a spatial and temporal manner. (© 2024. The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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