Salivary oxidative stress biomarkers in periodontitis-free smokers: a cross sectional study.

Autor:	Alrashdan MS; Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates - malrashdan@sharjah.ac.ae.; Department of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan - malrashdan@sharjah.ac.ae., Al-Shorman H; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Zarqa University, Zarqa, Jordan., Al-Dwairi A; Department of Physiology and Biochemistry, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan., Qutieshat A; Adult Restorative Dentistry, Oman Dental College, Muscat, Oman.; Honorary Researcher, Dundee Dental Hospital & School, Dundee, UK., Al-Omiri MK; Department of Prosthodontics, School of Dentistry, University of Jordan, Queen Rania Street, Amman, Jordan.; Department of Prosthodontics, The City of London School of Dentistry, London, UK.
Jazyk:	angličtina
Zdroj:	Minerva dental and oral science [Minerva Dent Oral Sci] 2024 Aug; Vol. 73 (4), pp. 209-216. Date of Electronic Publication: 2024 Jul 02.
DOI:	10.23736/S2724-6329.24.04879-4
Abstrakt:	Background: Salivary oxidative stress has been extensively studied with attempts to correlate changes in the oxidative stress markers with local and systemic factors, including smoking. The objective of this study was to evaluate the influence of two forms of smoking, cigarettes and waterpipe smoking (WPS), on selected oxidative stress biomarkers in saliva. Methods: Three groups of participants were enrolled into the study, controls (never smokers), cigarette smokers and WPS. Participants were clinically free from periodontitis and systemic conditions known to affect the saliva constituents. Unstimulated whole saliva samples were collected according to a standard protocol and concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG), myeloperoxidase (MPO), malondialdehyde (MDA), total antioxidant capacity (TAC), and cortisol. The one-way ANOVA test was used to compare the levels of each oxidative stress biomarker between the three study groups and the hierarchical linear regression analysis was used to test the levels of salivary cortisol for prediction of other oxidative stress biomarkers. Significance levels were set at 95% confidence intervals and probability values ≤0.05. Results: 8-OHdG was highest in WPS group (mean±SE 11,030.35±1829.16 pg/mL) while MDA and cortisol levels were highest in the cigarette smokers group (mean±SE 3.33±0.52 µM and 3.99±0.48 ng/mL, respectively) and MPO was highest in the control group (mean±SE 7.760±1.55 ng/mL). WPS group showed the highest TAC (mean±SE 0.3±0.03 mM). However, none of the tested makers reached a statistically significant difference. Conclusions: Despite subtle changes in some biomarkers, the salivary oxidative stress does not appear to be significantly influenced by smoking habits in periodontitis-free smokers.
Databáze:	MEDLINE
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