| Autor: |
Khalifa MM; Department of Medical Physiology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.; Department of Medical Physiology, College of Medicine, King Saud University, Kingdom of Saudi Arabia., Bastawy NA; Department of Medical Physiology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt., Rashed LA; Department of Biochemistry, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt., Hassan HA; Department of Histology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt., Abdel-Maksoud OM; Department of Medical Physiology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt., Hassan FE; Department of Medical Physiology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.; 5Department of Physiology, General Medicine Practice Program, Batterjee Medical College, Jeddah, Kingdom of Saudi Arabia. |
| Abstrakt: |
This study aimed to assess the prophylactic effects of Berberine on experimentally induced lung sepsis and examine its effects on selected cytokines, genes, and protein expression besides the histopathological evaluation. Berberine significantly reduced the wet/dry lung ratio, the broncho-alveolar lavage fluid (BALF) protein, cells, neutrophils percentage, and cytokines levels. In addition, pretreatment with Berberine decreased the myeloperoxidase (MPO) and malondialdehyde (MDA) levels and decreased gene expression of nuclear factor kappa B (NF-κB), monocyte chemoattractant protein-1 (MCP-1), and the intracellular adhesion molecule 1 (ICAM-1) by RT-qPCR analysis, revealing Berberine's antioxidant and anti-inflammatory mode of action. Western blot analysis revealed increased peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in the Berberine pretreated group compared to the cecal ligation and puncture (CLP) group, in which the histopathological examination evidenced this improvement. In conclusion, Berberine improved lung sepsis via its PPAR-γ mediated antioxidant and anti-inflammatory effects. |
