| Autor: |
Sun YQ; Graduate Training Base of Jinzhou Medical University (Linyi People's Hospital), Linyi 276000, China Linyi Key Laboratory of Tumor Biology, Linyi People's Hospital, Linyi 276000, China Department of Hematology, Linyi People's Hospital, Key Laboratory of Tumor Translational Medicine, Xuzhou Medical University, Linyi 276000, China., Fu Y; Linyi Key Laboratory of Tumor Biology, Linyi People's Hospital, Linyi 276000, China., Ji OX; Graduate Training Base of Jinzhou Medical University (Linyi People's Hospital), Linyi 276000, China., Wang LJ; Graduate Training Base of Jinzhou Medical University (Linyi People's Hospital), Linyi 276000, China Linyi Key Laboratory of Tumor Biology, Linyi People's Hospital, Linyi 276000, China Department of Hematology, Linyi People's Hospital, Key Laboratory of Tumor Translational Medicine, Xuzhou Medical University, Linyi 276000, China. |
| Abstrakt: |
The aim of this study was to investigate the effects of polyphyllin Ⅶ (PP Ⅶ) on proliferation, apoptosis, and cell cycle of diffuse large B-cell lymphoma (PLBCL) cell lines U2932 and SUDHL-4. The DLBCL cell lines were divided into a control group and a PPⅦ group, and experiments were conducted using MTT assay, flow cytometry, and Western blotting.Results showed that compared with the control group, PPⅦ significantly inhibited the proliferation of U2932 and SUDHL-4 cells ( P <0.05). Apoptosis assays demonstrated that treatment with 0.50 and 1.00 µmol/L PP Ⅶ significantly increased the apoptosis rates of both cell lines ( P <0.05), upregulated apoptosis-related proteins, and downregulated Bcl-2 protein level ( P <0.05). Cell cycle analysis revealed that PPⅦ treatment led to an increase in G0/G1-phase cells ( P <0.05) and a decrease in G2/M-phase cells ( P <0.05), significantly downregulated cyclin D1, CDK4, CDK6, and survivin protein expression ( P <0.05). In conclusion, PPⅦ exerted anti-lymphoma effects by inhibiting proliferation, promoting apoptosis, and inducing G0/G1 phase arrest in DLBCL cells. |
