Synthesis of non-modified near-infrared carbon dots for hypochlorite detection and cell membrane imaging.

Autor: Pang LF; College of Chemistry and Pharmaceutical Engineering, Huanghuai University, Zhumadian 463000, Henan, China. Electronic address: panglanfang1991@163.com., Fang WL; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China., Zhang B; College of Chemistry and Pharmaceutical Engineering, Huanghuai University, Zhumadian 463000, Henan, China., Xu QJ; College of Chemistry and Pharmaceutical Engineering, Huanghuai University, Zhumadian 463000, Henan, China. Electronic address: qijie001@163.com.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Nov 15; Vol. 321, pp. 124743. Date of Electronic Publication: 2024 Jun 27.
DOI: 10.1016/j.saa.2024.124743
Abstrakt: Devising carbon dots with long wavelength emission (red light or near infrared), high selectivity and good bio-compatibility is critical in fluorescence detection and imaging, but achieving this goal remains a great challenge. Herein, near-infrared emissive carbon dots (NIR-CDs) with obvious emission characteristic of 653 nm were synthesized through hydrothermally treatment of toluidine bule and gallic acid. Noticeably, the NIR-CDs exhibited excellent selectivity and sensitivity to hypochlorite (ClO - ), and the limit of detection is as low as 42.7 nM. The selective recognition reaction between ClO - and the surface functional groups of NIR-CDs inhibits the fluorescence from NIR-CDs. The quenching mechanism was confirmed by fluorescence lifetime decays, FT-IR spectroscopy and UV-vis absorption spectra. More remarkably, the NIR-CDs have rich hydrophilic groups showed lower cytotoxicity, excellent bio-compatibility and specific cell membrane localization ability. The established spectrofluorometric method based on NIR-CDs has been used to determination of ClO - level in tap water sample, the recoveries were 97.7 %-103.3 %. In addition, the NIR-CDs also has been successfully applied for the imaging of cell membrane. The study provides a novel idea for developing NIR ClO - probe as well as cell membrane localization probe based on CDs, which present bright prospects in real water samples monitoring and cell membrane imaging.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: