A catalogue of recombination coldspots in interspecific tomato hybrids.

Autor: Fuentes RR; Bioinformatics Group, Wageningen University and Research, Wageningen, The Netherlands.; Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany., Nieuwenhuis R; Business Unit of Bioscience, Cluster Applied Bioinformatics, Wageningen University and Research, Wageningen, The Netherlands., Chouaref J; Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.; Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands., Hesselink T; Business Unit of Bioscience, Cluster Applied Bioinformatics, Wageningen University and Research, Wageningen, The Netherlands., van Dooijeweert W; Centre for Genetic Resources, Wageningen University and Research, Wageningen, The Netherlands., van den Broeck HC; Business Unit of Bioscience, Cluster Applied Bioinformatics, Wageningen University and Research, Wageningen, The Netherlands., Schijlen E; Business Unit of Bioscience, Cluster Applied Bioinformatics, Wageningen University and Research, Wageningen, The Netherlands., Schouten HJ; Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands., Bai Y; Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands., Fransz P; Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Stam M; Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., de Jong H; Laboratory of Genetics, Wageningen University and Research, Wageningen, The Netherlands., Trivino SD; Business Unit of Bioscience, Cluster Applied Bioinformatics, Wageningen University and Research, Wageningen, The Netherlands., de Ridder D; Bioinformatics Group, Wageningen University and Research, Wageningen, The Netherlands., van Dijk ADJ; Bioinformatics Group, Wageningen University and Research, Wageningen, The Netherlands., Peters SA; Business Unit of Bioscience, Cluster Applied Bioinformatics, Wageningen University and Research, Wageningen, The Netherlands.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2024 Jul 01; Vol. 20 (7), pp. e1011336. Date of Electronic Publication: 2024 Jul 01 (Print Publication: 2024).
DOI: 10.1371/journal.pgen.1011336
Abstrakt: Increasing natural resistance and resilience in plants is key for ensuring food security within a changing climate. Breeders improve these traits by crossing cultivars with their wild relatives and introgressing specific alleles through meiotic recombination. However, some genomic regions are devoid of recombination especially in crosses between divergent genomes, limiting the combinations of desirable alleles. Here, we used pooled-pollen sequencing to build a map of recombinant and non-recombinant regions between tomato and five wild relatives commonly used for introgressive tomato breeding. We detected hybrid-specific recombination coldspots that underscore the role of structural variations in modifying recombination patterns and maintaining genetic linkage in interspecific crosses. Crossover regions and coldspots show strong association with specific TE superfamilies exhibiting differentially accessible chromatin between somatic and meiotic cells. About two-thirds of the genome are conserved coldspots, located mostly in the pericentromeres and enriched with retrotransposons. The coldspots also harbor genes associated with agronomic traits and stress resistance, revealing undesired consequences of linkage drag and possible barriers to breeding. We presented examples of linkage drag that can potentially be resolved by pairing tomato with other wild species. Overall, this catalogue will help breeders better understand crossover localization and make informed decisions on generating new tomato varieties.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Fuentes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje