Improved Diagnosis and Treatment Monitoring of Tuberculosis Using Stool and the Tuberculosis Bacterial Load Assay (TB-MBLA).

Autor:	Musisi E; Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK.; Infectious Diseases Research Collaboration, Kampala, Uganda.; Adroit Biomedical & Bioentreprenuership Research Services, Kampala, Uganda., Mtafya B; National Institute for Medical Research - Mbeya Medical Research Centre, Mbeya, Tanzania., Wambi WS; School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda., Zawedde J; Infectious Diseases Research Collaboration, Kampala, Uganda., Sessolo A; Infectious Diseases Research Collaboration, Kampala, Uganda., Ssengooba W; School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda., Walbaum N; Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK., Ntinginya NE; National Institute for Medical Research - Mbeya Medical Research Centre, Mbeya, Tanzania., Gillespie SH; Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK., Sabiiti W; Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK. ws31@st-andrews.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2833, pp. 153-160.
DOI:	10.1007/978-1-0716-3981-8_15
Abstrakt:	The diagnosis and monitoring of tuberculosis treatment is difficult as many patients are unable to produce sputum. This means that many patients are treated on the basis of clinical findings and consequently some will be exposed to anti-tuberculosis drugs unnecessarily. Moreover, for those appropriately on treatment and unable to produce a sputum sample, it will be impossible to monitor the response to treatment. We have shown that stool is a potential alternative sample type for diagnosis of tuberculosis. Currently, available protocols like the Xpert MTB/RIF use DNA as a target to detect Mycobacterium tuberculosis in stool but DNA survives long after the organism is dead so it is not certain whether a positive test is from an old or a partially treated infection. The TB MBLA only detects live organisms and thus, can be used to follow the response to treatment. In this chapter, we describe a protocol for TB-MBLA, an RNA-based assay, and apply it to quantify TB bacteria in stool. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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