HumanIslets: An integrated platform for human islet data access and analysis.

Autor:	Ewald JD; Institute of Parasitology, McGill University, Montreal, QC.; Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Lu Y; Institute of Parasitology, McGill University, Montreal, QC., Ellis CE; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB., Worton J; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Surgery, University of Alberta, Edmonton, AB., Kolic J; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC., Sasaki S; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada.; Department of Surgery, School of Biomedical Engineering, University of British Columbia, Vancouver, BC., Zhang D; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada.; Department of Surgery, School of Biomedical Engineering, University of British Columbia, Vancouver, BC., Dos Santos T; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB., Spigelman AF; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB., Bautista A; Alberta Diabetes Institute, University of Alberta, Edmonton, AB., Dai XQ; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB., Lyon JG; Alberta Diabetes Institute, University of Alberta, Edmonton, AB., Smith NP; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB., Wong JM; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Surgery, University of Alberta, Edmonton, AB., Rajesh V; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA., Sun H; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA., Sharp SA; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA., Rogalski JC; Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, Life Sciences Institute, University of British Columbia, Vancouver, BC., Moravcova R; Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, Life Sciences Institute, University of British Columbia, Vancouver, BC., Cen HH; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC., Manning Fox JE; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB., Atlas E; Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON., Bruin JE; Department of Biology & Institute of Biochemistry, Carleton University, Ottawa, ON., Mulvihill EE; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON.; University of Ottawa Heart Institute, Ottawa, ON., Verchere CB; Department of Surgery, BC Children's Hospital Research Institute and University of British Columbia, Vancouver, BC.; Department of Pathology and Laboratory Medicine, BC Children's Hospital Research Institute and University of British Columbia, Vancouver, BC.; Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, BC., Foster LJ; Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, Life Sciences Institute, University of British Columbia, Vancouver, BC., Gloyn AL; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA., Johnson JD; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC., Pepper AR; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Surgery, University of Alberta, Edmonton, AB., Lynn FC; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada.; Department of Surgery, School of Biomedical Engineering, University of British Columbia, Vancouver, BC., Xia J; Institute of Parasitology, McGill University, Montreal, QC., MacDonald PE; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.; Department of Pharmacology, University of Alberta, Edmonton, AB.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 22. Date of Electronic Publication: 2024 Jun 22.
DOI:	10.1101/2024.06.19.599613
Abstrakt:	Comprehensive molecular and cellular phenotyping of human islets can enable deep mechanistic insights for diabetes research. We established the Human Islet Data Analysis and Sharing (HI-DAS) consortium to advance goals in accessibility, usability, and integration of data from human islets isolated from donors with and without diabetes at the Alberta Diabetes Institute (ADI) IsletCore. Here we introduce HumanIslets.com, an open resource for the research community. This platform, which presently includes data on 547 human islet donors, allows users to access linked datasets describing molecular profiles, islet function and donor phenotypes, and to perform various statistical and functional analyses at the donor, islet and single-cell levels. As an example of the analytic capacity of this resource we show a dissociation between cell culture effects on transcript and protein expression, and an approach to correct for exocrine contamination found in hand-picked islets. Finally, we provide an example workflow and visualization that highlights links between type 2 diabetes status, SERCA3b Ca 2+ -ATPase levels at the transcript and protein level, insulin secretion and islet cell phenotypes. HumanIslets.com provides a growing and adaptable set of resources and tools to support the metabolism and diabetes research community. Competing Interests: Declaration of Interests ALG’s spouse is an employee of Genentech and holds stock options in Roche AP serves on the Scientific Advisory Committee for Encellin Inc. JX is founder of XiaLab Analytics, which provides omics data science training and support. All other authors confirm no relevant interests to declare.
Databáze:	MEDLINE
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