Effect of β-hydroxybutyrate acid on gene expression levels of antioxidant biomarkers and growth hormone-related genes in liver cell culture.

Autor:	Mohsin MA; Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China., Zhou X; Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China., Huiru Y; Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.; Shanghai Animal Disease Prevention and Control Center, Changning District, 201103, Shanghai, China., Shen W; Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China., He B; Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China., Sobiech P; Department of Clinical Sciences, Internal Disease Unit, Faculty of Veterinary Medicine, Utrecht, Netherlands., Pierzchała M; Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, 05-552 Magdalenka, Poland., Ogłuszka M; Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, 05-552 Magdalenka, Poland., Starzyński R; Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, 05-552 Magdalenka, Poland., Kalra G; Department of Animal Genetics and Breeding, Ludhiana, Punjab, India., Deshmukh B; Department of Animal Genetics and Breeding, Ludhiana, Punjab, India., Thangarasu R; College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India., Kashyap N; College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India., Czarnik U; Department of Pig Breeding, Faculty of Animal Bio-Engineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland., Lepczyński A; Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 71-270 Szczecin, Poland., Woźniakowski G; Department of Infectious and Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Toruń, Poland.; Division of Functional Genomics in Biological and Biomedical Research, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 87-100 Toruń, Poland., Pareek CS; Department of Infectious and Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Toruń, Poland.; Division of Functional Genomics in Biological and Biomedical Research, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 87-100 Toruń, Poland.
Jazyk:	angličtina
Zdroj:	Journal of veterinary research [J Vet Res] 2024 Jun 22; Vol. 68 (2), pp. 313-324. Date of Electronic Publication: 2024 Jun 22 (Print Publication: 2024).
DOI:	10.2478/jvetres-2024-0037
Abstrakt:	Introduction: In dairy cattle, oxidative stress is a predominant problem associated with diseases and reproductive health issues. This study aimed to detect the variation in the antioxidant biomarkers by adding different concentrations of β-hydroxybutyric acid (BHBA) and sought to elucidate its effects on the gene expression levels of growth hormone (GH) and antioxidant biomarkers in bovine hepatocytes. Material and Methods: Four antioxidant biomarkers, namely malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) were evaluated using commercially available bovine ELISA kits. The expression levels of the bovine GH, its receptor (GHR), insulin-like growth factor (IGF), IGF-1, IGF-1 receptor, CAT, SOD, GSH-Px and β-actin (as a reference) genes in liver cell culture were determined by reverse transcriptase-PCR assay. Results: With the increase of BHBA concentration and culture time, the activities of SOD, CAT, and GSH Px biomarkers in hepatocytes decreased. However, the content of MDA in hepatocytes increased gradually with the increase of hepatocyte culture time and BHBA concentration. The qPCR results revealed that after adding BHBA, gene expression levels of GSH-Px, SOD and IGF biomarkers in hepatocytes began to differ in the culture groups at 12 h, whereas the gene expression level of the CAT and GHR biomarkers in hepatocytes began to differ at 6 h. Conclusion: Quantitative PCR results showed that the BHBA significantly downregulated the expression levels of the GHR gene and CAT, GSH Px and SOD antioxidant biomarker genes. Competing Interests: Conflict of Interests Statement: The authors declare there is no conflict of interests regarding the publication of this article. (© 2024 Muhammad Ali Mohsin et al., published by Sciendo.)
Databáze:	MEDLINE
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