How does a Saccharomyces cerevisiae extract influence the components of isolated rotavirus particles from stool samples collected in a clinical setting from children?
| Autor: | Hussein MAM; Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt., Al-Zaban MI; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia., Mahmoud YAG; Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt., Al-Doaiss AA; Biology Department, College of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia., Bahshwan SMA; Biological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia., El-Dougdoug KA; Microbiology Department, Faculty of Agriculture, Ain Shams University, PO Box 68, Hadayek Shobra 11241, Cairo, Egypt., El-Shanshory MR; Pediatric Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt. |
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| Jazyk: | angličtina |
| Zdroj: | Saudi journal of biological sciences [Saudi J Biol Sci] 2024 Aug; Vol. 31 (8), pp. 104031. Date of Electronic Publication: 2024 May 26. |
| DOI: | 10.1016/j.sjbs.2024.104031 |
| Abstrakt: | Human Rotavirus (HRV) is the causative pathogen of severe acute enteric infections that cause mortality among children worldwide. This study focuses on developing a new and effective treatment for rotavirus infection using an extract from Saccharomyces cerevisiae, aiming to make this treatment easily accessible to everyone. 15 antigens and 26 antibodies were detected in serum and stool using ELISA. The titers of HRVq1, HRVq2, HRVC1, and HRVC2 on Vero cells were determined to be 1.2x10 6 , 3.0x10 6 , 4.2x10 6 , and 7.5x10 5 (Plaque forming unit, PFU/ml) four days after infection, respectively. The HRVq1 isolate induced cytopathic effects, i.e., forming multinucleated, rounded, enlarged, and expanding gigantic cells. RT-PCR identified this isolate, and the accession number 2691714 was assigned to GeneBank. The molecular docking analysis revealed that nonstructural proteins (NSPs) NSP1, NSP2, NSP3, NSP4, NSP5, and NSP6 exhibited significant binding with RNA. NSP2 demonstrated the highest binding affinity and the lowest binding energy (-8.9 kcal/mol). This affinity was maintained via hydrophobic interactions and hydrogen bonds spanning in length from 1.12 Å to 3.11 Å. The ADMET and bioactivity predictions indicated that the yeast extract possessed ideal solubility, was nontoxic, and did not cause cancer. The inhibitory constant values predicted for the S. cerevisiae extract in the presence of HRV vital proteins varied from 5.32 to 7.45 mM, indicating its potential as a viable drug candidate. Saccharomyces cerevisiae extract could be utilized as a dietary supplement to combat HRV as an alternative dietary supplement. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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