JRK binds satellite III DNA and is necessary for the heat shock response.

Autor: Waldron R; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland., Rodriguez MLAB; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland., Williams JM; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland., Ning Z; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland., Ahmed A; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland., Lindsay A; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland., Moore T; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2024 Aug; Vol. 48 (8), pp. 1212-1222. Date of Electronic Publication: 2024 Jul 01.
DOI: 10.1002/cbin.12216
Abstrakt: JRK is a DNA-binding protein of the pogo superfamily of transposons, which includes the well-known centromere binding protein B (CENP-B). Jrk null mice exhibit epilepsy, and growth and reproductive disorders, consistent with its relatively high expression in the brain and reproductive tissues. Human JRK DNA variants and gene expression levels are implicated in cancers and neuropsychiatric disorders. JRK protein modulates β-catenin-TCF activity but little is known of its cellular functions. Based on its homology to CENP-B, we determined whether JRK binds centromeric or other satellite DNAs. We show that human JRK binds satellite III DNA, which is abundant at the chromosome 9q12 juxtacentromeric region and on Yq12, both sites of nuclear stress body assembly. Human JRK-GFP overexpressed in HeLa cells strongly localises to 9q12. Using an anti-JRK antiserum we show that endogenous JRK co-localises with a subset of centromeres in non-stressed cells, and with heat shock factor 1 following heat shock. Knockdown of JRK in HeLa cells proportionately reduces heat shock protein gene expression in heat-shocked cells. A role for JRK in regulating the heat shock response is consistent with the mouse Jrk null phenotype and suggests that human JRK may act as a modifier of diseases with a cellular stress component.
(© 2024 The Author(s). Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology.)
Databáze: MEDLINE
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