Inhibitor of cardiolipin biosynthesis-related enzyme MoGep4 confers broad-spectrum anti-fungal activity.

Autor: Sun P; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Zhao J; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Sha G; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Zhou Y; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Zhao M; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Li R; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Kong X; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Sun Q; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Li Y; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Li K; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Bi R; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Yang L; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Qin Z; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Huang W; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Wang Y; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Gao J; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Chen G; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Zhang H; Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China., Adnan M; Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, China., Yang L; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Zheng L; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Chen XL; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China., Wang G; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China., Ishikawa T; Graduate School of Science and Engineering, Saitama University, Saitama, Japan., Li Q; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Xu JR; Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana, USA., Li G; National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center of Crop Nanotechnology, Huazhong Agricultural University, Wuhan, China.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Nov; Vol. 47 (11), pp. 4259-4274. Date of Electronic Publication: 2024 Jul 01.
DOI: 10.1111/pce.15021
Abstrakt: Plant pathogens cause devastating diseases, leading to serious losses to agriculture. Mechanistic understanding of pathogenesis of plant pathogens lays the foundation for the development of fungicides for disease control. Mitophagy, a specific form of autophagy, is important for fungal virulence. The role of cardiolipin, mitochondrial signature phospholipid, in mitophagy and pathogenesis is largely unknown in plant pathogenic fungi. The functions of enzymes involved in cardiolipin biosynthesis and relevant inhibitors were assessed using a set of assays, including genetic deletion, plant infection, lipidomics, chemical-protein interaction, chemical inhibition, and field trials. Our results showed that the cardiolipin biosynthesis-related gene MoGEP4 of the rice blast fungus Magnaporthe oryzae regulates growth, conidiation, cardiolipin biosynthesis, and virulence. Mechanistically, MoGep4 regulated mitophagy and Mps1-MAPK phosphorylation, which are required for virulence. Chemical alexidine dihydrochloride (AXD) inhibited the enzyme activity of MoGep4, cardiolipin biosynthesis and mitophagy. Importantly, AXD efficiently inhibited the growth of 10 plant pathogens and controlled rice blast and Fusarium head blight in the field. Our study demonstrated that MoGep4 regulates mitophagy, Mps1 phosphorylation and pathogenesis in M. oryzae. In addition, we found that the MoGep4 inhibitor, AXD, displays broad-spectrum antifungal activity and is a promising candidate for fungicide development.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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