Confocal laser scanning evaluation of the influence of ledges on root canal disinfection.

Autor: Berutti E; Department of Surgical Science, CIR-Dental School, University of Turin, Turin, Italy., Pasqualini D; Department of Surgical Science, CIR-Dental School, University of Turin, Turin, Italy., Scotti N; Department of Surgical Science, CIR-Dental School, University of Turin, Turin, Italy., Mandras N; Department of Public Health and Pediatrics, University of Turin, Turin, Italy., Roana J; Department of Public Health and Pediatrics, University of Turin, Turin, Italy., Carpegna G; Department of Surgical Science, CIR-Dental School, University of Turin, Turin, Italy., Palopoli P; Department of Surgical Science, CIR-Dental School, University of Turin, Turin, Italy., Alovisi M; Department of Surgical Science, CIR-Dental School, University of Turin, Turin, Italy.
Jazyk: angličtina
Zdroj: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc [Aust Endod J] 2024 Dec; Vol. 50 (3), pp. 580-586. Date of Electronic Publication: 2024 Jun 30.
DOI: 10.1111/aej.12871
Abstrakt: The aim was to analyse the influence of an apical ledge on root canal disinfection. Forty-four single-rooted teeth were micro-CT scanned and inoculated with Enterococcus faecalis. In Group S shaping was performed with ProTaper Next (PTN) up to X3 at working length (WL). In Group L an apical ledge was created with K-Files #40 and shaping completed up to PTN X3. NaOCl 5% and EDTA 10% irrigant solutions were alternated. Confocal laser scanning microscope (CLSM) and viability staining were used to analyse the proportions of dead (red) and live (green) bacteria and penetration ability inside dentinal tubules. Data were analysed with the Mann-Whitney test with Bonferroni correction (p < 0.05). In Group L the amount of red fluorescence resulted significantly lower, and penetration ability was decreased in the apical and middle portion (p < 0.05). The presence of an apical ledge may negatively influence the disinfection both in the apical and middle third.
(© 2024 Australian Society of Endodontology Inc.)
Databáze: MEDLINE
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