CLEM, a universal tool for analyzing structural organization in thylakoid membranes.

Autor: Lübben MK; Department of Molecular Plant Science, LMU Munich, Planegg-Martinsried, Germany., Klingl A; Plant Development, LMU Munich, Planegg-Martinsried, Germany., Nickelsen J; Department of Molecular Plant Science, LMU Munich, Planegg-Martinsried, Germany., Ostermeier M; Department of Molecular Plant Science, LMU Munich, Planegg-Martinsried, Germany.
Jazyk: angličtina
Zdroj: Physiologia plantarum [Physiol Plant] 2024 Jul-Aug; Vol. 176 (4), pp. e14417.
DOI: 10.1111/ppl.14417
Abstrakt: Chlorophyll (Chl) plays a crucial role in photosynthesis, functioning as a photosensitizer. As an integral component of this process, energy absorbed by this pigment is partly emitted as red fluorescence. This signal can be readily imaged by fluorescence microscopy and provides a visualization of photosynthetic activity. However, due to limited resolution, signals cannot be assigned to specific subcellular/organellar membrane structures. By correlating fluorescence micrographs with transmission electron microscopy, researchers can identify sub-cellular compartments and membranes, enabling the monitoring of Chl distribution within thylakoid membrane substructures in cyanobacteria, algae, and higher plant single cells. Here, we describe a simple and effective protocol for correlative light-electron microscopy (CLEM) based on the autofluorescence of Chl and demonstrate its application to selected photosynthetic model organisms. Our findings illustrate the potential of this technique to identify areas of high Chl concentration and photochemical activity, such as grana regions in vascular plants, by mapping stacked thylakoids.
(© 2024 The Author(s). Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.)
Databáze: MEDLINE
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