Construction of a high-density linkage map and QTL detection for growth traits in South African abalone (Haliotis midae).

Autor: Tshilate TS; Department of Genetics, Stellenbosch University, Matieland, South Africa., Ishengoma E; Department of Genetics, Stellenbosch University, Matieland, South Africa.; Mkwawa University College of Education, University of Dar es Salaam, Iringa, Tanzania., Rhode C; Department of Genetics, Stellenbosch University, Matieland, South Africa.
Jazyk: angličtina
Zdroj: Animal genetics [Anim Genet] 2024 Oct; Vol. 55 (5), pp. 744-760. Date of Electronic Publication: 2024 Jun 30.
DOI: 10.1111/age.13462
Abstrakt: Haliotis midae is one of the most important molluscs in South African commercial aquaculture. In this study, a high-resolution integrated linkage map was constructed, and QTL identified using 2b-RADseq for genotyping SNPs in three families. The final integrated linkage map was composed by merging the individual family maps, resulting in 3290 informative SNPs mapping to 18 linkage groups, conforming to the known haploid chromosome number for H. midae. The total map spanned 1798.25 cM with an average marker interval of 0.55 cM, representing a genome coverage of 98.76%. QTL analysis, across all three families, resulted in a total of five QTL identified for growth-related traits, shell width, shell length, and total body weight. For shell width and total body weight, one QTL was identified for each trait respectively, whilst three QTL were identified for shell length. The identified QTL respectively explained between 7.20% and 11.40% of the observed phenotypic variance. All three traits were significantly correlated (r = 0.862-0.970; p < 0.01) and shared overlapping QTL. The QTL for growth traits were mapped back to the H. midae draft genome and BLAST searches revealed the identity of candidate genes, such as egf-1, megf10, megf6, tnx, sevp1, kcp, notch1, and scube2 with possible functional roles in H. midae growth. The constructed high-density linkage map and mapped QTL have given valuable insights regarding the genetic architecture of growth-related traits and will be important genetic resources for marker-assisted selection. It remains, however, important to validate causal variants through linkage disequilibrium fine mapping in future.
(© 2024 The Author(s). Animal Genetics published by John Wiley & Sons Ltd on behalf of Stichting International Foundation for Animal Genetics.)
Databáze: MEDLINE
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