Individual virome analysis reveals the general co-infection of mammal-associated viruses with SARS-related coronaviruses in bats.

Autor:	Si HR; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China; University of Chinese Academy of Sciences, Beijing 100000, China., Wu K; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, China., Su J; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China; University of Chinese Academy of Sciences, Beijing 100000, China., Dong TY; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China; University of Chinese Academy of Sciences, Beijing 100000, China., Zhu Y; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China., Li B; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China., Chen Y; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China., Li Y; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China., Shi ZL; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43000, China; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, China. Electronic address: zlshi@wh.iov.cn., Zhou P; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, China; State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical School, Guangzhou 510005, China. Electronic address: zhou_peng@gzlab.ac.cn.
Jazyk:	angličtina
Zdroj:	Virologica Sinica [Virol Sin] 2024 Aug; Vol. 39 (4), pp. 565-573. Date of Electronic Publication: 2024 Jun 28.
DOI:	10.1016/j.virs.2024.06.008
Abstrakt:	Bats are the natural reservoir hosts for SARS-related coronavirus (SARSr-CoV) and other highly pathogenic microorganisms. Therefore, it is conceivable that an individual bat may harbor multiple microbes. However, there is limited knowledge on the overall co-circulation of microorganisms in bats. Here, we conducted a 16-year monitoring of bat viruses in south and central China and identified 238 SARSr-CoV positive samples across nine bat species from ten provinces or administrative districts. Among these, 76 individual samples were selected for further metagenomics analysis. We found a complex microenvironment characterized by the general co-circulation of microbes from two different sources: mammal-associated viruses or environment-associated microbes. The later includes commensal bacteria, enterobacteria-related phages, and insect or fungal viruses of food origin. Results showed that 25% (19/76) of the samples contained at least one another mammal-associated virus, notably alphacoronaviruses (13/76) such as AlphaCoV/YN2012, HKU2-related CoV and AlphaCoV/Rf-HuB2013, along with viruses from other families. Notably, we observed three viruses co-circulating within a single bat, comprising two coronavirus species and one picornavirus. Our analysis also revealed the potential presence of pathogenic bacteria or fungi in bats. Furthermore, we obtained 25 viral genomes from the 76 bat SARSr-CoV positive samples, some of which formed new evolutionary lineages. Collectively, our study reveals the complex microenvironment of bat microbiome, facilitating deeper investigations into their pathogenic potential and the likelihood of cross-species transmission. Competing Interests: Conflict of interest Prof. Zheng-Li Shi is the Editor-in-Chief for Virologica Sinica and Prof. Peng Zhou is an editorial board member for Virologica Sinica. They were not involved in the editorial review or the decision to publish this article. The authors declare that they have no competing interests. (Copyright © 2024 The Authors. Publishing services by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu