White light increases anticancer effectiveness of iridium(III) complexes toward lung cancer A549 cells.

Autor: Li G; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Chen J; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Xie Y; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Yang Y; Department of Pharmacy, Guangdong Second Provincial General Hospital, 510317, PR China. Electronic address: yany@gd2h.org.cn., Niu Y; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Chen X; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Zeng X; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Zhou L; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China., Liu Y; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. Electronic address: lyjche@gdpu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of inorganic biochemistry [J Inorg Biochem] 2024 Oct; Vol. 259, pp. 112652. Date of Electronic Publication: 2024 Jun 26.
DOI: 10.1016/j.jinorgbio.2024.112652
Abstrakt: Anticancer activity has been extensively studies. In this article, three ligands 2-(6-bromobenzo[d][1,3]dioxol-5-yl)-1H-imidazo[4,5-f][1,10]phenanthroline (BDIP), 2-(7-methoxybenzo[d][1,3]dioxol-5-yl)-1H-imidazo[4,5-f][1,10]phenanthroline (MDIP), 2-(6-nitrobenzo[d][1,3]dioxol-5-yl)-1H-imidazo[4,5-f][1,10]phenanthroline (NDIP) and their iridium(III) complexes: [Ir(ppy) 2 (BDIP)](PF 6 ) (ppy = deprotonated 2-phenylpyridine, 3a), [Ir(ppy) 2 (MDIP)](PF 6 ) (3b) and [Ir(ppy) 2 (NDIP)](PF 6 ) (3c) were synthesized. The cytotoxicity of 3a, 3b, 3c against Huh7, A549, BEL-7402, HepG2, HeLa, and non-cancer NIH3T3 was tested using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. The results obtained from the MTT test stated clearly that these complexes demonstrated moderate or non-cytotoxicity toward Huh7, BEL-7402, HepG2 and HeLa except A549 cells. To improve the anticancer efficacy, we used white light to irradiate the mixture of cells and complexes for 30 min, the anticancer activity of the complexes was greatly enhanced. Particularly, 3a and 3b exhibited heightened capability to inhibit A549 cells proliferation with IC 50 (half maximal inhibitory concentration) values of 0.7 ± 0.3 μM and 1.8 ± 0.1 μM, respectively. Cellular uptake has shown that 3a and 3b can be accumulated in the cytoplasm. Wound healing and colony forming showed that 3a and 3b significantly hinder the cell migration and growth in the S phase. The complexes open mitochondrial permeability transition pore (MPTP) channel and cause the decrease of membrane potential, release of cytochrome C, activation of caspase 3, and finally lead to apoptosis. In addition, 3a and 3b cause autophagy, increase the lipid peroxidation and lead to ferroptosis. Also, 3a and 3b increase the expression of calreticulin (CRT), high mobility group box 1 (HMGB1), heat shock protein 70 (HSP70), thereby inducing immunogenic cell death.
Competing Interests: Declaration of competing interest The authors declare no competing interest exists.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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