A comprehensive gene expression analysis of the unique three-layered cocoon of the cecropia moth, Hyalophora cecropia.

Autor: Rouhová L; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic., Podlahová Š; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic., Kmet P; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic., Žurovec M; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic. Electronic address: zurovec@entu.cas.cz., Sehadová H; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic. Electronic address: sehadova@entu.cas.cz., Sauman I; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic. Electronic address: sauman@entu.cas.cz.
Jazyk: angličtina
Zdroj: Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2024 Aug; Vol. 171, pp. 104152. Date of Electronic Publication: 2024 Jun 27.
DOI: 10.1016/j.ibmb.2024.104152
Abstrakt: The larvae of the moth Hyalophora cecropia spin silk cocoons with morphologically distinct layers. We investigated the expression of the individual silk protein components of these cocoons in relation to the morphology of the silk gland and its affiliation to the different layers of the cocoon. The study used transcriptomic and proteomic analyses to identify 91 proteins associated with the silk cocoons, 63 of which have a signal peptide indicating their secretory nature. We checked the specificity of their expression in different parts of the SG and the presence of the corresponding protein products in each cocoon layer. Differences were observed among less abundant proteins with unclear functions. The representation of proteins in the inner envelope and intermediate space was similar, except for a higher proportion of probable contaminating proteins, mostly originating from the gut. On the other hand, the outer envelope contains a number of putative enzymes with unclear function. However, the protein most specific to the outer layer has sequence homology to putative serine/threonine kinase-like proteins and some adhesive proteins, and its closest homolog in Bombyx mori was found in the scaffold silk. This research provides valuable insights into the silk production of the cecropia moth, highlighting both similarities and differences to other moth species.
Competing Interests: Declaration of Competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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