Loxosceles amazonica Brown Spider venom: Insights into enzymatic activities, immunorecognition, and novel phospholipase D isoforms.

Autor: Silva-Magalhães R; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil; Biochemistry and Immunology Department, Biological Sciences Institute, Federal University of Minas Gerais - UFMG, Belo Horizonte, MG, Brazil., Silva-Araújo AL; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Peres-Damásio P; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Teixeira Pereira EH; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., de Oliveira Souza R; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Varela LSDRN; Arachnid Proteomics Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Tomé LMR; Central Laboratory of Public Health of Minas Gerais, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., de Melo Iani FC; Central Laboratory of Public Health of Minas Gerais, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Silveira AL; Biótica Estudos Ambientais, João Pinheiro, MG, Brazil., Borges MH; Arachnid Proteomics Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Medina-Santos R; Biochemistry and Immunology Department, Biological Sciences Institute, Federal University of Minas Gerais - UFMG, Belo Horizonte, MG, Brazil., Chavez-Olórtegui C; Biochemistry and Immunology Department, Biological Sciences Institute, Federal University of Minas Gerais - UFMG, Belo Horizonte, MG, Brazil., Vasconcelos Diniz MR; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Paiva ALB; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil., Guerra-Duarte C; Molecular Toxinology Lab, Research and Development Department, Ezequiel Dias Foundation - Funed, Belo Horizonte, MG, Brazil. Electronic address: clara.duarte@funed.mg.gov.br.
Jazyk: angličtina
Zdroj: Biochimie [Biochimie] 2024 Jun 27. Date of Electronic Publication: 2024 Jun 27.
DOI: 10.1016/j.biochi.2024.06.012
Abstrakt: The Loxosceles genus represents one of the main arachnid genera of medical importance in Brazil. Despite the gravity of Loxosceles-related accidents, just a handful of species are deemed medically important and only a few have undergone comprehensive venom characterization. Loxosceles amazonica is a notable example of a potentially dangerous yet understudied Loxosceles species. While there have been limited reports of accidents involving L. amazonica to date, accidents related to Loxosceles are increasing in the North and Northeast regions of Brazil, where L. amazonica has been reported. In this work, we provide a complementary biochemical and immunological characterization of L. amazonica venom, considering its most relevant enzymatic activities and its immunorecognition and neutralization by current therapeutic antivenoms. Additionally, a cDNA library enriched with phospholipase D (PLD) sequences from L. amazonica venom glands was built and subsequently sequenced. The results showed that L. amazonica venom is well immunorecognised by all the tested antibodies. Its venom also displayed proteolytic, hyaluronidase, and sphingomyelinase activities. These activities were at least partially inhibited by available antivenoms. With cDNA sequencing of PLDs, seven new putative isoforms were identified in the venom of L. amazonica. These results contribute to a better knowledge of the venom content and activities of a synanthropic, yet understudied, Loxosceles species. In vivo assays are essential to confirm the medical relevance of L. amazonica, as well as to assess its true toxic potential and elucidate its related pathophysiology.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
Databáze: MEDLINE
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