Vitrification of pig embryos dysregulates the microRNA transcriptome profile.

Autor: Cuello C; Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain., González-Plaza A; Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain. Electronic address: alejandro.gonzalez9@um.es., Cambra JM; Large Animal Models in Cardiovascular Research, Internal Medical Department I, TUMunich, Technical University of Munich, Munich, Germany., Garcia-Canovas M; Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain., Parrilla I; Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain., Rodriguez-Martinez H; Department of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, SE-58185 Linköping, Sweden., Gil MA; Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain., Martinez EA; Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', Institute for Biomedical Research of Murcia (IMIB-Arrixaca), University of Murcia, 30100, Murcia, Spain.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Sep 15; Vol. 226, pp. 243-252. Date of Electronic Publication: 2024 Jun 14.
DOI: 10.1016/j.theriogenology.2024.06.001
Abstrakt: This study examined how the vitrification of pig blastocysts using either the superfine open pulled straw (SOPS) or Cryotop method affects the expression profile of embryonic microRNA (miRNA) transcriptomes, as well as its relation to changes in the expression of target genes (TGs). Surgically collected pig blastocysts were vitrified using either the SOPS method (n = 60; 4-6 embryos/device) or the Cryotop system (n = 60; 20 embryos/device). Embryos were cultured in vitro for 24 h after warming. Fresh blastocysts (n = 60) cultured for 24 h served as controls. After in vitro culture, five pools of eight viable blastocysts from each group were prepared for miRNA expression analysis based on a microarray approach. Then, biological interpretation of miRNAs profiles and integrative analysis of miRNA and mRNA transcriptome data were performed. Survival after 24 h of in vitro culture was similar (>96 %) for both the vitrification systems and the control group (100 %). Compared with the controls, the SOPS-vitrified blastocysts had 94 (one upregulated and 93 downregulated) differentially expressed (DE) miRNAs, and the Cryotop-vitrified blastocysts had 174 DE miRNAs (one upregulated and 173 downregulated). One DE miRNA (miR-503) in the SOPS group and three DE miRNAs (miR-7139-3p, miR-214 and miR-885-3p) in the Cryotop group were annotated for Sus scrofa. The integrative analysis showed that 27 and 61 DE TGs were regulated by the DE miRNAs in blastocysts vitrified with the SOPS and Cryotop systems, respectively. The TGs enriched one pathway (the TGF-β signaling pathway) for the SOPS system and four pathways (HIF-1, Notch, ascorbate and aldarate metabolism and glycosphingolipid biosynthesis-ganglio series) for the Cryotop system. In summary, vitrification via the SOPS and Cryotop systems dysregulates miRNAs, with slight differences between methods. The altered miRNAs identified in this study were related mainly to cell proliferation, apoptosis, and the response to cell stress. Further studies are needed to clarify the consequences of dysregulation of miRNAs involved in the TGF-β (SOPS-vitrified blastocyst) and Notch (Cryotop-vitrified blastocyst) signaling pathways, particularly if they can affect embryonic development.
Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartially of the research reported.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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