Protocol for detecting mitochondria extracellular vesicles of brown adipose tissue in mice.
| Autor: | Tortolici F; University of Rome, 'Tor Vergata,' Biology Department, 00133 Rome, Italy., Di Biagio C; University of Rome, 'Tor Vergata,' Biology Department, 00133 Rome, Italy., Lettieri-Barbato D; University of Rome, 'Tor Vergata,' Biology Department, 00133 Rome, Italy. Electronic address: daniele.lettieribarbato@uniroma2.it., Aquilano K; University of Rome, 'Tor Vergata,' Biology Department, 00133 Rome, Italy. Electronic address: katia.aquilano@uniroma2.it. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103161. Date of Electronic Publication: 2024 Jun 28. |
| DOI: | 10.1016/j.xpro.2024.103161 |
| Abstrakt: | Brown adipose tissue (BAT) is mitochondria rich, enabling high oxidative metabolism for non-shivering thermogenesis. The release of large/small extracellular vesicles (EVs) containing mitochondria or mitochondrial fragments, termed mito-EVs, may support mitochondrial quality control or intercellular communication. We present a protocol to isolate and characterize mito-EVs. We detail steps for BAT processing, cell debris removal, differential centrifugation (dC), and mito-EV analysis by flow cytometry and immunoblotting assays. For complete details on the use and execution of this protocol, please refer to Rosina et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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