Liangxue Tongyu prescription attenuates neuroinflammation by increasing cholecystokinin octapeptide in acute intracerebral hemorrhage rats.

Autor: Li J; School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Department of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210022, China., Sun Y; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China., Qiu W; School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China., Zhou Y; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China., Zhou D; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China., Zhao Y; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China., Liu A; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China., Yuan Y; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China. Electronic address: ioiyy@njucm.edu.cn., Guo W; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China. Electronic address: gwfwfg2003@njucm.edu.cn.
Jazyk: angličtina
Zdroj: Neuropeptides [Neuropeptides] 2024 Oct; Vol. 107, pp. 102452. Date of Electronic Publication: 2024 Jun 24.
DOI: 10.1016/j.npep.2024.102452
Abstrakt: Inflammatory reactions after acute intracerebral hemorrhage (AICH) contribute significantly to a poor prognosis. Liangxue Tongyu Prescription (LTP) has been proven to be clinically effective in treating AICH. Numerous studies have shown that LTP suppresses brain inflammatory damage in AICH, while the internal mechanisms underlying its action remain unclear. The aim of this study was to verify the anti-inflammatory effects of LTP on an AICH rat model and investigate the potential mechanisms. The AICH rat models were created by injecting autologous blood into the right caudate nucleus. LTP markedly decreased cerebral hematoma and brain water content and recovered from neurological deficits. Meanwhile, LTP prevented microglial activation and reduced the inflammatory reaction caused by pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Notably, the expression of cholecystokinin octapeptide (CCK-8) in the brain and intestine was increased by LTP or CCK-8 treatment. LTP further suppressed nuclear factor kappa B (NF-κB) in the brains of rats with AICH. Moreover, LTP increased the protein and mRNA expression of Occludin and Claudin-1 in the intestine and decreased the levels of lipopolysaccharide (LPS) and diamine oxidase (DAO) in serum. Furthermore, the results showed that LTP increased the protein and mRNA expression of Claudin-5 and zonula occludens-1 (ZO-1) in the brain. CCK-8 receptor antagonists increased the expression of NF-κB and the concentration of pro-inflammatory cytokines. These findings suggested that LTP attenuated neuroinflammation by increasing CCK-8 in the brain and intestine, and its mechanism might be related to alterations in the gut-brain axis (GBA).
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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